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Evapotranspiration (ET) plays a key role in water resource management. It is important to understand the ET spatio-temporal pattern of South Asia for understanding and anticipating serious water resource shortages. In this study, daily ET in 2008 was estimated over South Asia by using MODerate Resolution Imaging Spectroradiometer (MODIS) products combined with field observations and Global Land Data Assimilation System (GLDAS) product through Surface Energy Balance System (SEBS) model. Monthly ET data were calculated based on daily ET and evaluated by the GLDAS ET data. Good agreements were found between two datasets for winter months (October to February) with R-2 from 0.5 to 0.7. Spatio-temporal analysis of ET was conducted. Ten specific sites with different land cover types at typical climate regions were selected to analyze the ET temporal change pattern, and the result indicated that the semi-arid or arid areas in the northwest had the lowest average daily ET (around 0.3 mm) with a big fluctuation in the monsoon season, while the sites in the Indo-Gangetic Plain and in southern India has bigger daily ET (more than 3 mm) due to a large water supplement. It is suggested that the monsoon climate has a large impact on ET spatio-temporal variation in the whole region.