干旱区生态环境知识资源中心

The aim of this study was to investigate the mechanism by the comparison with germinability and water imbibition between non-scarified and scarified seeds. The seed structure was studied illustrated through SEM and the effect of pre-soaking and soaked solution on seed germination was determined with GA(3). Germination percentage was significantly improved (98.5%) in scarified seeds on 25 degrees C in dark, whereas the value in non-scarified seeds was only 27.5%. Water absorption percentage in scarified seeds was higher (41.55%) than that of non-scarified seeds (36.68%). From seed illustration, the micropyle was jammed by some waxy substance which impeded water into seeds and the hard seed coat made up of collenchyma and palisade tissue which led to the barrier to water permeability. Germination percentage wasn’t significantly improved (from 27.5% to 48.5%) by pre-soaking with the solution of 500mg/l GA(3), whereas significantly improved (from 27.5% to 98.5%) in the same concentration of GA(3). Our results showed that seed dormancy in Corispermum patelliforme Iljin seeds was caused by the hard seed coat, the jammed micropyle and the inhibitory compounds from the " bubble structure". It can be concluded that dormancy-releasing requires removing or destroying seed coat.