Arid
DOI10.1007/s40009-014-0288-6
Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea
Bohra, Abhishek1; Singh, I. P.1; Yadav, Ashutosh K.2; Pathak, Abhinav3; Soren, K. R.1,4; Chaturvedi, S. K.1; Nadarajan, N.1
通讯作者Bohra, Abhishek
来源期刊NATIONAL ACADEMY SCIENCE LETTERS-INDIA
ISSN0250-541X
EISSN2250-1754
出版年2015
卷号38期号:1页码:13-19
英文摘要

Pigeonpea is one of the most important pulse crops in the semi-arid tropical region, which is prone to several climatic uncertainties like unpredictable temperature, frequent drought and inconsistent rainfall. Additionally, during crop cycle pigeonpea also encounters a wide range of other biotic and abiotic constraints, ultimately leading to its fluctuating production and stagnant productivity. However, recently developed CGMS system has shown noteworthy impacts in enhancing pigeonpea productivity through exploitation of hybrid vigour. At present, A(2)-cytoplasm derived CGMS system has been well established in pigeonpea. Nevertheless, the commercial success of CGMS system relies largely on the continuous supply of genetically pure seeds of hybrids and corresponding parental lines. Traditionally, the genetic purity of seeds is guaranteed through conducting grow out test (GoT). In this context, DNA marker assays offer several advantages over conventional GoT especially in terms of time, space and money. Given its locus-specific and co-dominant nature, SSR or microsatellite marker is particularly suited for hybridity testing and purity assessment. Here we report a set of robust SSR markers, which could act as reliable molecular kit for ensuring the genetic purity of the CGMS-hybrid ’IPH 09-5’ and its parental lines ’PA 163A’ (A-or Male sterile-line) and ’AK 261322’ (R- or Restorer-line).


英文关键词Pigeonpea Hybrid GoT Molecular marker SSR Polymorphism
类型Article
语种英语
国家India
收录类别SCI-E
WOS记录号WOS:000348140600003
WOS关键词CAJANUS-CAJAN ; MICROSATELLITE MARKERS ; PURITY ASSESSMENT ; WILD RELATIVES ; L. ; DIVERSITY ; SUNFLOWER ; FREQUENCY ; SEQUENCE ; MAP
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/189297
作者单位1.Indian Inst Pulses Res, Crop Improvement Div, Kanpur 208024, Uttar Pradesh, India;
2.Indian Agr Res Inst, Div Genet, New Delhi 110012, India;
3.Natl Bur Fish Genet Resources, Lucknow 226002, Uttar Pradesh, India;
4.Indian Inst Pulses Res, Biotechnol Unit, Kanpur 208024, Uttar Pradesh, India
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GB/T 7714
Bohra, Abhishek,Singh, I. P.,Yadav, Ashutosh K.,et al. Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea[J],2015,38(1):13-19.
APA Bohra, Abhishek.,Singh, I. P..,Yadav, Ashutosh K..,Pathak, Abhinav.,Soren, K. R..,...&Nadarajan, N..(2015).Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea.NATIONAL ACADEMY SCIENCE LETTERS-INDIA,38(1),13-19.
MLA Bohra, Abhishek,et al."Utility of Informative SSR Markers in the Molecular Characterization of Cytoplasmic Genetic Male Sterility-Based Hybrid and its Parents in Pigeonpea".NATIONAL ACADEMY SCIENCE LETTERS-INDIA 38.1(2015):13-19.
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