Arid
DOI10.1111/1755-0998.12335
Genetic identification of endangered North African ungulates using noninvasive sampling
Silva, Teresa Luisa1,2,3; Godinho, Raquel1,2; Castro, Diana1; Abaigar, Teresa3; Brito, Jose Carlos1,2; Alves, Paulo Celio1,2,4
通讯作者Silva, Teresa Luisa
来源期刊MOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
EISSN1755-0998
出版年2015
卷号15期号:3页码:652-661
英文摘要

North African ungulates include several threatened and emblematic species, yet are poorly studied mainly due to their remoteness and elusiveness. Noninvasive sampling provides a useful approach to obtain ecological and genetic information essential to guide conservation actions. The very first and most important step in conservation planning is to accurately identify species, and molecular genetics has been proved to be a useful tool. Several molecular genetics protocols are available for species identification, even for samples with poor quality DNA, such as faeces, hairs or bones. Most of these protocols use mitochondrial DNA for barcoding despite this marker being especially prone to problems, including mtDNA introgression, nuclear insert copies, high intraspecific diversity or heteroplasmy. In this work, we developed a molecular method based on polymorphisms in small fragments of the mitochondrial cytochrome b (cytb, mtDNA) and the nuclear kappa casein genes (KCAS, nDNA) for identifying endangered North African ungulates. These fragments revealed polymorphisms, including species-specific variation, which allowed species identification of nine ungulate species that co-occur in North Africa. The method was validated across more than 400 samples, including different types of noninvasive samples collected in the field. The simplicity, high reliability and relative low cost of the described method make it a promising tool to improve ecological studies of the North African ungulates and consequently, the implementation of more efficient management and conservation plans for these endangered ungulates.


英文关键词conservation genetics cytochrome b deserts gazelles kappa casein molecular method species ID
类型Article
语种英语
国家Portugal ; Spain ; USA
收录类别SCI-E
WOS记录号WOS:000352653700017
WOS关键词GENOTYPING ERROR RATES ; MITOCHONDRIAL-DNA ; BIODIVERSITY ASSESSMENT ; MOUNTAIN GAZELLES ; DORCAS GAZELLE ; CYTOCHROME-B ; CONSERVATION ; EVOLUTION ; BOVIDAE ; HYBRIDIZATION
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/189251
作者单位1.Univ Porto, CIBIO InBIO, Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Vairao, Portugal;
2.Univ Porto, Dept Biol, Fac Ciencias, P-4169007 Oporto, Portugal;
3.CSIC, EEZA, Almeria, Spain;
4.Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA
推荐引用方式
GB/T 7714
Silva, Teresa Luisa,Godinho, Raquel,Castro, Diana,et al. Genetic identification of endangered North African ungulates using noninvasive sampling[J],2015,15(3):652-661.
APA Silva, Teresa Luisa,Godinho, Raquel,Castro, Diana,Abaigar, Teresa,Brito, Jose Carlos,&Alves, Paulo Celio.(2015).Genetic identification of endangered North African ungulates using noninvasive sampling.MOLECULAR ECOLOGY RESOURCES,15(3),652-661.
MLA Silva, Teresa Luisa,et al."Genetic identification of endangered North African ungulates using noninvasive sampling".MOLECULAR ECOLOGY RESOURCES 15.3(2015):652-661.
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