Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1111/1755-0998.12335 |
Genetic identification of endangered North African ungulates using noninvasive sampling | |
Silva, Teresa Luisa1,2,3; Godinho, Raquel1,2; Castro, Diana1; Abaigar, Teresa3; Brito, Jose Carlos1,2; Alves, Paulo Celio1,2,4 | |
通讯作者 | Silva, Teresa Luisa |
来源期刊 | MOLECULAR ECOLOGY RESOURCES
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ISSN | 1755-098X |
EISSN | 1755-0998 |
出版年 | 2015 |
卷号 | 15期号:3页码:652-661 |
英文摘要 | North African ungulates include several threatened and emblematic species, yet are poorly studied mainly due to their remoteness and elusiveness. Noninvasive sampling provides a useful approach to obtain ecological and genetic information essential to guide conservation actions. The very first and most important step in conservation planning is to accurately identify species, and molecular genetics has been proved to be a useful tool. Several molecular genetics protocols are available for species identification, even for samples with poor quality DNA, such as faeces, hairs or bones. Most of these protocols use mitochondrial DNA for barcoding despite this marker being especially prone to problems, including mtDNA introgression, nuclear insert copies, high intraspecific diversity or heteroplasmy. In this work, we developed a molecular method based on polymorphisms in small fragments of the mitochondrial cytochrome b (cytb, mtDNA) and the nuclear kappa casein genes (KCAS, nDNA) for identifying endangered North African ungulates. These fragments revealed polymorphisms, including species-specific variation, which allowed species identification of nine ungulate species that co-occur in North Africa. The method was validated across more than 400 samples, including different types of noninvasive samples collected in the field. The simplicity, high reliability and relative low cost of the described method make it a promising tool to improve ecological studies of the North African ungulates and consequently, the implementation of more efficient management and conservation plans for these endangered ungulates. |
英文关键词 | conservation genetics cytochrome b deserts gazelles kappa casein molecular method species ID |
类型 | Article |
语种 | 英语 |
国家 | Portugal ; Spain ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000352653700017 |
WOS关键词 | GENOTYPING ERROR RATES ; MITOCHONDRIAL-DNA ; BIODIVERSITY ASSESSMENT ; MOUNTAIN GAZELLES ; DORCAS GAZELLE ; CYTOCHROME-B ; CONSERVATION ; EVOLUTION ; BOVIDAE ; HYBRIDIZATION |
WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/189251 |
作者单位 | 1.Univ Porto, CIBIO InBIO, Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Vairao, Portugal; 2.Univ Porto, Dept Biol, Fac Ciencias, P-4169007 Oporto, Portugal; 3.CSIC, EEZA, Almeria, Spain; 4.Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA |
推荐引用方式 GB/T 7714 | Silva, Teresa Luisa,Godinho, Raquel,Castro, Diana,et al. Genetic identification of endangered North African ungulates using noninvasive sampling[J],2015,15(3):652-661. |
APA | Silva, Teresa Luisa,Godinho, Raquel,Castro, Diana,Abaigar, Teresa,Brito, Jose Carlos,&Alves, Paulo Celio.(2015).Genetic identification of endangered North African ungulates using noninvasive sampling.MOLECULAR ECOLOGY RESOURCES,15(3),652-661. |
MLA | Silva, Teresa Luisa,et al."Genetic identification of endangered North African ungulates using noninvasive sampling".MOLECULAR ECOLOGY RESOURCES 15.3(2015):652-661. |
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