Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1111/mec.13106 |
Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis | |
Rzotkiewicz, Sabine1; Gutierrez, Ricardo1; Krasnov, Boris R.2; Morick, Danny1; Khokhlova, Irina S.3; Nachum-Biala, Yaarit1; Baneth, Gad1; Harrus, Shimon1 | |
通讯作者 | Harrus, Shimon |
来源期刊 | MOLECULAR ECOLOGY
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ISSN | 0962-1083 |
EISSN | 1365-294X |
出版年 | 2015 |
卷号 | 24期号:6页码:1364-1373 |
英文摘要 | Fleas are acknowledged vectors and reservoirs of various bacteria that present a wide range of pathogenicity. In this study, fleas collected from wild rodents from the Negev desert in southern Israel were tested for RickettsiaDNA by targeting the 16S rRNA (rrs) gene. Thirty-eight Xenopsylla ramesis, 91 Synosternus cleopatrae and 15 Leptopsylla flea pools (a total of 568 fleas) were screened. RickettsiaDNA was detected in 100% of the X.ramesis and in one S.cleopatrae flea pools. None of L.algira flea pools was found positive. All positive flea pools were further characterized by sequencing of five additional genetic loci (gltA, ompB, ompA, htrA and fusA). The molecular identification of the positive samples showed all sequences to be closely related to the Rickettsia felis-like’ organisms (99-100% similarities in the six loci). To further investigate the association between R.felis-like’ and X.ramesis fleas, ten additional single X.ramesis adult fleas collected from the wild and five laboratory-maintained X.ramesis imago, five larva pools (2-18 larvae per pool) and two egg pools (18 eggs per pool) were tested for the presence of R.felis-like’ DNA. All samples were found positive by a specific ompAPCR assay, confirming the close association of this Rickettsia species with X.ramesis in all its life stages. These results suggest a symbiotic association between Rickettsia felis-like’ and X.ramesis fleas. |
英文关键词 | Candidatus Rickettsia asemboensis endosymbiosis Rickettsia felis Rickettsia felis-like |
类型 | Article |
语种 | 英语 |
国家 | Israel |
收录类别 | SCI-E |
WOS记录号 | WOS:000351465300015 |
WOS关键词 | SPOTTED-FEVER GROUP ; MURINE TYPHUS ; MOLECULAR-DETECTION ; NEGEV DESERT ; CAT FLEAS ; GENOTYPIC IDENTIFICATION ; SIBIRICA-MONGOLITIMONAE ; CTENOCEPHALIDES-FELIS ; ISRAEL ; BARTONELLA |
WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
来源机构 | Ben-Gurion University of the Negev ; Hebrew University of Jerusalem |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/189241 |
作者单位 | 1.Hebrew Univ Jerusalem, Koret Sch Vet Med, IL-76100 Rehovot, Israel; 2.Ben Gurion Univ Negev, Wyler Dept Dryland Agr, French Associates Inst Agr & Biotechnol Drylands, Jacob Blaustein Inst Desert Res, IL-84990 Midreshet Ben Gurion, Israel; 3.Ben Gurion Univ Negev, Inst Dryland Environm Res, Jacob Blaustein Inst Desert Res, Mitrani Dept Desert Ecol, IL-84990 Midreshet Ben Gurion, Israel |
推荐引用方式 GB/T 7714 | Rzotkiewicz, Sabine,Gutierrez, Ricardo,Krasnov, Boris R.,等. Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis[J]. Ben-Gurion University of the Negev, Hebrew University of Jerusalem,2015,24(6):1364-1373. |
APA | Rzotkiewicz, Sabine.,Gutierrez, Ricardo.,Krasnov, Boris R..,Morick, Danny.,Khokhlova, Irina S..,...&Harrus, Shimon.(2015).Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis.MOLECULAR ECOLOGY,24(6),1364-1373. |
MLA | Rzotkiewicz, Sabine,et al."Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis".MOLECULAR ECOLOGY 24.6(2015):1364-1373. |
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