Arid
DOI10.1111/mec.13106
Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis
Rzotkiewicz, Sabine1; Gutierrez, Ricardo1; Krasnov, Boris R.2; Morick, Danny1; Khokhlova, Irina S.3; Nachum-Biala, Yaarit1; Baneth, Gad1; Harrus, Shimon1
通讯作者Harrus, Shimon
来源期刊MOLECULAR ECOLOGY
ISSN0962-1083
EISSN1365-294X
出版年2015
卷号24期号:6页码:1364-1373
英文摘要

Fleas are acknowledged vectors and reservoirs of various bacteria that present a wide range of pathogenicity. In this study, fleas collected from wild rodents from the Negev desert in southern Israel were tested for RickettsiaDNA by targeting the 16S rRNA (rrs) gene. Thirty-eight Xenopsylla ramesis, 91 Synosternus cleopatrae and 15 Leptopsylla flea pools (a total of 568 fleas) were screened. RickettsiaDNA was detected in 100% of the X.ramesis and in one S.cleopatrae flea pools. None of L.algira flea pools was found positive. All positive flea pools were further characterized by sequencing of five additional genetic loci (gltA, ompB, ompA, htrA and fusA). The molecular identification of the positive samples showed all sequences to be closely related to the Rickettsia felis-like’ organisms (99-100% similarities in the six loci). To further investigate the association between R.felis-like’ and X.ramesis fleas, ten additional single X.ramesis adult fleas collected from the wild and five laboratory-maintained X.ramesis imago, five larva pools (2-18 larvae per pool) and two egg pools (18 eggs per pool) were tested for the presence of R.felis-like’ DNA. All samples were found positive by a specific ompAPCR assay, confirming the close association of this Rickettsia species with X.ramesis in all its life stages. These results suggest a symbiotic association between Rickettsia felis-like’ and X.ramesis fleas.


英文关键词Candidatus Rickettsia asemboensis endosymbiosis Rickettsia felis Rickettsia felis-like
类型Article
语种英语
国家Israel
收录类别SCI-E
WOS记录号WOS:000351465300015
WOS关键词SPOTTED-FEVER GROUP ; MURINE TYPHUS ; MOLECULAR-DETECTION ; NEGEV DESERT ; CAT FLEAS ; GENOTYPIC IDENTIFICATION ; SIBIRICA-MONGOLITIMONAE ; CTENOCEPHALIDES-FELIS ; ISRAEL ; BARTONELLA
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
来源机构Ben-Gurion University of the Negev ; Hebrew University of Jerusalem
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/189241
作者单位1.Hebrew Univ Jerusalem, Koret Sch Vet Med, IL-76100 Rehovot, Israel;
2.Ben Gurion Univ Negev, Wyler Dept Dryland Agr, French Associates Inst Agr & Biotechnol Drylands, Jacob Blaustein Inst Desert Res, IL-84990 Midreshet Ben Gurion, Israel;
3.Ben Gurion Univ Negev, Inst Dryland Environm Res, Jacob Blaustein Inst Desert Res, Mitrani Dept Desert Ecol, IL-84990 Midreshet Ben Gurion, Israel
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GB/T 7714
Rzotkiewicz, Sabine,Gutierrez, Ricardo,Krasnov, Boris R.,等. Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis[J]. Ben-Gurion University of the Negev, Hebrew University of Jerusalem,2015,24(6):1364-1373.
APA Rzotkiewicz, Sabine.,Gutierrez, Ricardo.,Krasnov, Boris R..,Morick, Danny.,Khokhlova, Irina S..,...&Harrus, Shimon.(2015).Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis.MOLECULAR ECOLOGY,24(6),1364-1373.
MLA Rzotkiewicz, Sabine,et al."Novel evidence suggests that a ’Rickettsia felis-like’ organism is an endosymbiont of the desert flea, Xenopsylla ramesis".MOLECULAR ECOLOGY 24.6(2015):1364-1373.
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