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Behavioural fever is a widely conserved response to infection. The host increases body temperature (T-b) by altering their preferred temperature (T-p), generating a fever and delaying or avoiding pathogen-induced mortality. This response is not ubiquitous in insects, however, although few studies have investigated this response to viral infection. Here, we examined the change in T-p of Drosophila in response to virus infection using a thermal gradient. No difference in T-p was observed. We suggest that the lack of behavioural fever could be due to the increased energy cost of maintaining a higher T-b whilst the immune response is active. To the best of our knowledge, this is the first study to assay for changes in T-p of infected Drosophila.