Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1093/jxb/erv191 |
Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana | |
Abraham Juarez, Maria Jazmn1; Hernandez Cardenas, Rocio1; Santoyo Villa, Jose Natzul1; O’Connor, Devin2; Sluis, Aaron3; Hake, Sarah3; Ordaz-Ortiz, Jose4; Terry, Leon4; Simpson, June1 | |
通讯作者 | Simpson, June |
来源期刊 | JOURNAL OF EXPERIMENTAL BOTANY
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ISSN | 0022-0957 |
EISSN | 1460-2431 |
出版年 | 2015 |
卷号 | 66期号:13页码:3893-3905 |
英文摘要 | In Agave tequilana, reproductive failure or inadequate flower development stimulates the formation of vegetative bulbils at the bracteoles, ensuring survival in a hostile environment. Little is known about the signals that trigger this probably unique phenomenon in agave species. Here we report that auxin plays a central role in bulbil development and show that the localization of PIN1-related proteins is consistent with altered auxin transport during this process. Analysis of agave transcriptome data led to the identification of the A. tequilana orthologue of PIN1 (denoted AtqPIN1) and a second closely related gene from a distinct clade reported as ’sister of PIN1’ (denoted AtqSoPIN1). Quantitative real-time reverse transcription-PCR (RT-qPCR) analysis showed different patterns of expression for each gene during bulbil formation, and heterologous expression of the A. tequilana PIN1 and SoPIN1 genes in Arabidopsis thaliana confirmed functional differences between these genes. Although no free auxin was detected in induced pedicel samples, changes in the levels of auxin precursors were observed. Taken as a whole, the data support the model that AtqPIN1 and AtqSoPIN1 have co-ordinated but distinct functions in relation to auxin transport during the initial stages of bulbil formation. |
英文关键词 | Agave tequilana auxin flux bulbil development gene expression immunolocalization PIN proteins |
类型 | Article |
语种 | 英语 |
国家 | Mexico ; England ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000358533400019 |
WOS关键词 | MEXICAN TROPICAL DESERT ; MACROACANTHA AGAVACEAE ; POLLINATION ECOLOGY ; EXPRESSION ; PATTERNS ; GENES ; PLANT ; METABOLISM ; MECHANISMS ; CYTOKININ |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
来源机构 | University of California, Berkeley |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/188611 |
作者单位 | 1.Cinvestav Irapuato, Dept Plant Genet Engn, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Guanajuato 36821, Mexico; 2.Univ Cambridge, Sainsbury Lab, Cambridge CB2 1LR, England; 3.Univ Calif Berkeley, Ctr Plant Gene Express, USDA ARS, Plant & Microbial Biol Dept, Berkeley, CA 94720 USA; 4.Cranfield Univ, Plant Sci Lab, Cranfield MK43 0AL, Beds, England |
推荐引用方式 GB/T 7714 | Abraham Juarez, Maria Jazmn,Hernandez Cardenas, Rocio,Santoyo Villa, Jose Natzul,et al. Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana[J]. University of California, Berkeley,2015,66(13):3893-3905. |
APA | Abraham Juarez, Maria Jazmn.,Hernandez Cardenas, Rocio.,Santoyo Villa, Jose Natzul.,O’Connor, Devin.,Sluis, Aaron.,...&Simpson, June.(2015).Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana.JOURNAL OF EXPERIMENTAL BOTANY,66(13),3893-3905. |
MLA | Abraham Juarez, Maria Jazmn,et al."Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana".JOURNAL OF EXPERIMENTAL BOTANY 66.13(2015):3893-3905. |
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