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DOI | 10.1242/jcs.176057 |
Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization | |
Kanemoto, Soshi1; Kobayashi, Yasuhiro2; Yamashita, Teruhito2; Miyamoto, Takeshi3; Cui, Min1; Asada, Rie1; Cui, Xiang1; Hino, Kenta1; Kaneko, Masayuki1; Takai, Tomoko1; Matsuhisa, Koji1; Takahashi, Naoyuki2; Imaizumi, Kazunori1 | |
通讯作者 | Kanemoto, Soshi |
来源期刊 | JOURNAL OF CELL SCIENCE
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ISSN | 0021-9533 |
EISSN | 1477-9137 |
出版年 | 2015 |
卷号 | 128期号:23页码:4353-4365 |
英文摘要 | Luman (also known as CREB3) is a type-II transmembrane transcription factor belonging to the OASIS family that localizes to the endoplasmic reticulum (ER) membrane under normal conditions. In response to ER stress, OASIS-family members are subjected to regulated intramembrane proteolysis (RIP), following which the cleaved N-terminal fragments translocate to the nucleus. In this study, we show that treatment of bone marrow macrophages (BMMs) with cytokines - macrophage colony-stimulating factor (M-CSF) and RANKL (also known as TNFSF11) - causes a time-dependent increase in Luman expression, and that Luman undergoes RIP and becomes activated during osteoclast differentiation. Small hairpin (sh) RNA-mediated knockdown of Luman in BMMs prevented the formation of multinucleated osteoclasts, concomitant with the suppression of DC-STAMP, a protein that is essential for cell-cell fusion in osteoclastogenesis. The N-terminus of Luman facilitates promoter activity of DC-STAMP, resulting in upregulation of DC-STAMP expression. Furthermore, Luman interacts with DC-STAMP, and controls its stability and localization. These results suggest that Luman regulates the multinucleation of osteoclasts by promoting cell fusion of mononuclear osteoclasts through DC-STAMP induction and intracellular distribution during osteoclastogenesis. |
英文关键词 | Osteoclastogenesis Cell-cell fusion Endoplasmic reticulum Transcription factor |
类型 | Article |
语种 | 英语 |
国家 | Japan |
收录类别 | SCI-E |
WOS记录号 | WOS:000366316400013 |
WOS关键词 | CELL-CELL FUSION ; BODY GIANT-CELLS ; ENDOPLASMIC-RETICULUM ; ER STRESS ; TRANSMEMBRANE PROTEIN ; SIGNALING MECHANISMS ; BONE-FORMATION ; TRANSCRIPTION ; ACTIVATION ; NFATC1 |
WOS类目 | Cell Biology |
WOS研究方向 | Cell Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/188453 |
作者单位 | 1.Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Biochem, Minami Ku, Hiroshima 7348553, Japan; 2.Matsumoto Dent Univ, Inst Oral Sci, Nagano 3990781, Japan; 3.Keio Univ, Sch Med, Dept Orthoped Surg, Shinjuku Ku, Tokyo 1608582, Japan |
推荐引用方式 GB/T 7714 | Kanemoto, Soshi,Kobayashi, Yasuhiro,Yamashita, Teruhito,et al. Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization[J],2015,128(23):4353-4365. |
APA | Kanemoto, Soshi.,Kobayashi, Yasuhiro.,Yamashita, Teruhito.,Miyamoto, Takeshi.,Cui, Min.,...&Imaizumi, Kazunori.(2015).Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization.JOURNAL OF CELL SCIENCE,128(23),4353-4365. |
MLA | Kanemoto, Soshi,et al."Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization".JOURNAL OF CELL SCIENCE 128.23(2015):4353-4365. |
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