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DOI10.1242/jcs.176057
Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization
Kanemoto, Soshi1; Kobayashi, Yasuhiro2; Yamashita, Teruhito2; Miyamoto, Takeshi3; Cui, Min1; Asada, Rie1; Cui, Xiang1; Hino, Kenta1; Kaneko, Masayuki1; Takai, Tomoko1; Matsuhisa, Koji1; Takahashi, Naoyuki2; Imaizumi, Kazunori1
通讯作者Kanemoto, Soshi
来源期刊JOURNAL OF CELL SCIENCE
ISSN0021-9533
EISSN1477-9137
出版年2015
卷号128期号:23页码:4353-4365
英文摘要

Luman (also known as CREB3) is a type-II transmembrane transcription factor belonging to the OASIS family that localizes to the endoplasmic reticulum (ER) membrane under normal conditions. In response to ER stress, OASIS-family members are subjected to regulated intramembrane proteolysis (RIP), following which the cleaved N-terminal fragments translocate to the nucleus. In this study, we show that treatment of bone marrow macrophages (BMMs) with cytokines - macrophage colony-stimulating factor (M-CSF) and RANKL (also known as TNFSF11) - causes a time-dependent increase in Luman expression, and that Luman undergoes RIP and becomes activated during osteoclast differentiation. Small hairpin (sh) RNA-mediated knockdown of Luman in BMMs prevented the formation of multinucleated osteoclasts, concomitant with the suppression of DC-STAMP, a protein that is essential for cell-cell fusion in osteoclastogenesis. The N-terminus of Luman facilitates promoter activity of DC-STAMP, resulting in upregulation of DC-STAMP expression. Furthermore, Luman interacts with DC-STAMP, and controls its stability and localization. These results suggest that Luman regulates the multinucleation of osteoclasts by promoting cell fusion of mononuclear osteoclasts through DC-STAMP induction and intracellular distribution during osteoclastogenesis.


英文关键词Osteoclastogenesis Cell-cell fusion Endoplasmic reticulum Transcription factor
类型Article
语种英语
国家Japan
收录类别SCI-E
WOS记录号WOS:000366316400013
WOS关键词CELL-CELL FUSION ; BODY GIANT-CELLS ; ENDOPLASMIC-RETICULUM ; ER STRESS ; TRANSMEMBRANE PROTEIN ; SIGNALING MECHANISMS ; BONE-FORMATION ; TRANSCRIPTION ; ACTIVATION ; NFATC1
WOS类目Cell Biology
WOS研究方向Cell Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/188453
作者单位1.Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Biochem, Minami Ku, Hiroshima 7348553, Japan;
2.Matsumoto Dent Univ, Inst Oral Sci, Nagano 3990781, Japan;
3.Keio Univ, Sch Med, Dept Orthoped Surg, Shinjuku Ku, Tokyo 1608582, Japan
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Kanemoto, Soshi,Kobayashi, Yasuhiro,Yamashita, Teruhito,et al. Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization[J],2015,128(23):4353-4365.
APA Kanemoto, Soshi.,Kobayashi, Yasuhiro.,Yamashita, Teruhito.,Miyamoto, Takeshi.,Cui, Min.,...&Imaizumi, Kazunori.(2015).Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization.JOURNAL OF CELL SCIENCE,128(23),4353-4365.
MLA Kanemoto, Soshi,et al."Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization".JOURNAL OF CELL SCIENCE 128.23(2015):4353-4365.
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