干旱区生态环境知识资源中心

A halotolerant, Gram-negative, rod-shaped and light-red-pigmented bacterium, designated LP51(T), was isolated from pond sediment near a hexachlorocyclohexane dumpsite located at Chinhat, Lucknow, India. Phylogenetic analysis based on 1 6S rRNA gene sequences showed that strain LP51(T) formed a distinct phyletic clade along with the members of the genus Pontibacter. The 16S rRNA gene sequence similarity to members of the genus Pontibacter ranged from 94.2 to 99.4%. The cells were motile, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C-15:0 (17.8%), iso-C-15:0 3-OH (8.8%), iso-C-17:0 3-OH (5.7 %), summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c; 6.5 %) and summed feature 4 (iso-C-17:1 I and/or anteiso-C-17:1 B; 30.7%). The polar lipid profile of strain LP51(T) showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids, unknown polar lipids and unknown glycolipids. DNA DNA relatedness of strain LP51T with respect to the most closely related type strain, Pontibacter korlensis X14-1(T), was 47.2%. On the basis of this information, it is proposed that the isolate be assigned to a novel species of the genus Pontibacter, for which the name Pontibacter chinhatensis sp. nov. is proposed. The type strain is LP51(T) (=CCM 8436(T)=MCC 2070(T)).