Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3390/ijms160920468 |
Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms | |
Wang, Hou-Ling1,2; Li, Lan1; Tang, Sha3; Yuan, Chao1; Tian, Qianqian1; Su, Yanyan1; Li, Hui-Guang1; Zhao, Lin1; Yin, Weilun1,2; Zhao, Rui1; Xia, Xinli1 | |
通讯作者 | Zhao, Rui |
来源期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
ISSN | 1422-0067 |
出版年 | 2015 |
卷号 | 16期号:9页码:20468-20491 |
英文摘要 | Despite the unshakable status of reverse transcription-quantitative PCR in gene expression analysis, it has certain disadvantages, including that the results are highly dependent on the reference genes selected for data normalization. Since inappropriate endogenous control genes will lead to inaccurate target gene expression profiles, the validation of suitable internal reference genes is essential. Given the increasing interest in functional genes and genomics of Populus euphratica, a desert poplar showing extraordinary adaptation to salt stress, we evaluated the expression stability of ten candidate reference genes in P. euphratica roots, stems, and leaves under salt stress conditions. We used five algorithms, namely, C-t, NormFinder, geNorm, GrayNorm, and a rank aggregation method (RankAggreg) to identify suitable normalizers. To support the suitability of the identified reference genes and to compare the relative merits of these different algorithms, we analyzed and compared the relative expression levels of nine P. euphratica functional genes in different tissues. Our results indicate that a combination of multiple reference genes recommended by GrayNorm algorithm (e.g., a combination of Actin, EF1, GAPDH, RP, UBQ in root) should be used instead of a single reference gene. These results are valuable for research of gene identification in different P. euphratica tissues. |
英文关键词 | reference genes salt stress normalization reverse transcription-quantitative PCR Populus euphratica |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000364541000024 |
WOS关键词 | REAL-TIME PCR ; RELIABLE REFERENCE GENES ; POPULUS-EUPHRATICA ; RT-PCR ; EXPRESSION ANALYSIS ; HOUSEKEEPING GENES ; SALINITY TOLERANCE ; INTERNAL CONTROL ; STORAGE PROTEIN ; LEAF SUCCULENCE |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
来源机构 | 北京林业大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/188022 |
作者单位 | 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China; 2.Beijing Forestry Univ, Coll Forestry, Minist Educ, Key Lab Silviculture & Conservat, Beijing 100083, Peoples R China; 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Hou-Ling,Li, Lan,Tang, Sha,et al. Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms[J]. 北京林业大学,2015,16(9):20468-20491. |
APA | Wang, Hou-Ling.,Li, Lan.,Tang, Sha.,Yuan, Chao.,Tian, Qianqian.,...&Xia, Xinli.(2015).Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,16(9),20468-20491. |
MLA | Wang, Hou-Ling,et al."Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 16.9(2015):20468-20491. |
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