Arid
DOI10.3389/fnins.2015.00084
Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria
Lismont, Els1; Vleugels, Rut1; Marchal, Elisabeth1,2; Badisco, Liesbeth1; Van Wielendaele, Pieter1; Lenaerts, Cynthia1; Zels, Sven1; Tobe, Stephen S.2; Broeck, Jozef Vanden1; Verlinden, Heleen1
通讯作者Verlinden, Heleen
来源期刊FRONTIERS IN NEUROSCIENCE
ISSN1662-453X
出版年2015
卷号9
英文摘要

Allatotropins (ATs) are pleiotropic neuropeptides initially isolated from the tobacco hornworm, Manduca sexta. In 2008, the first receptor for AT-like peptides (ATR) was characterized in Bombyx mori. Since then, ATRs have also been characterized in M. sexta, Tribolium castaneum, Aedes aegypti and Bombus terrestris. These receptors show sequence similarity to vertebrate orexin (ORX) receptors. When generating an EST-database of the desert locust (Schistocerca gregaria) central nervous system, we found cDNA sequences encoding the Schgr-AT precursor and a fragment of its putative receptor. This receptor cDNA has now been completed and functionally expressed in mammalian cell lines. Activation of this receptor, designated as Schgr-ATR, by Schgr-AT caused an increase in intracellular calcium ions, as well as cyclic AMP (cAMP), with an EC50 value in the nanomolar range. In addition, the transcript distribution of both the Schgr-AT precursor and Schgr-ATR was investigated by means of quantitative real-time PCR. Moreover, we found more evidence for the myotropic and allatostimulatory actions of Schgr-AT in the desert locust. These data are discussed and situated in a broader context by comparison with literature data on AT and ATR in insects.


英文关键词GPCR insect juvenile hormone neuropeptide orexin peptide motility
类型Article
语种英语
国家Belgium ; Canada
收录类别SCI-E
WOS记录号WOS:000352968700001
WOS关键词MANDUCA-SEXTA ALLATOTROPIN ; JUVENILE-HORMONE BIOSYNTHESIS ; LOM-AG-MYOTROPIN ; CORPORA ALLATA ; TRIBOLIUM-CASTANEUM ; FUNCTIONAL-CHARACTERIZATION ; SPODOPTERA-FRUGIPERDA ; SIGNALING PROPERTIES ; FRONTAL GANGLION ; GENE-EXPRESSION
WOS类目Neurosciences
WOS研究方向Neurosciences & Neurology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/187388
作者单位1.Katholieke Univ Leuven, Mol Dev Physiol & Signal Transduct, B-3000 Leuven, Belgium;
2.Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada
推荐引用方式
GB/T 7714
Lismont, Els,Vleugels, Rut,Marchal, Elisabeth,et al. Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria[J],2015,9.
APA Lismont, Els.,Vleugels, Rut.,Marchal, Elisabeth.,Badisco, Liesbeth.,Van Wielendaele, Pieter.,...&Verlinden, Heleen.(2015).Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria.FRONTIERS IN NEUROSCIENCE,9.
MLA Lismont, Els,et al."Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria".FRONTIERS IN NEUROSCIENCE 9(2015).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lismont, Els]的文章
[Vleugels, Rut]的文章
[Marchal, Elisabeth]的文章
百度学术
百度学术中相似的文章
[Lismont, Els]的文章
[Vleugels, Rut]的文章
[Marchal, Elisabeth]的文章
必应学术
必应学术中相似的文章
[Lismont, Els]的文章
[Vleugels, Rut]的文章
[Marchal, Elisabeth]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。