干旱区生态环境知识资源中心(Arid): A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage

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DOI	10.1093/femsle/fnv110
	A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage
	Anderson, Dominique 1; Ferreras, Eloy 2; Trindade, Marla 1; Cowan, Don 1,2
通讯作者	Ferreras, Eloy
来源期刊	FEMS MICROBIOLOGY LETTERS
ISSN	0378-1097
EISSN	1574-6968
出版年	2015
卷号	362 期号:15
英文摘要	Metagenomic library screening, by functional or sequence analysis, has become an established method for the identification of novel genes and gene products, including genetic elements implicated in microbial stress response and adaptation. We have identified, using a sequence-based approach, a fosmid clone from an Antarctic desert soil metagenome library containing a novel gene which codes for a protein homologous to a Water Hypersensitivity domain (WHy). The WHy domain is typically found as a component of specific LEA (Late Embryogenesis Abundant) proteins, particularly the LEA-14 (LEA-8) variants, which occur widely in plants, nematodes, bacteria and archaea and which are typically induced by exposure to stress conditions. The novel WHy-like protein (165 amino acid, 18.6 kDa) exhibits a largely invariant NPN motif at the N-terminus and has high sequence identity to genes identified in Pseudomonas genomes. Expression of this protein in Escherichia coli significantly protected the recombinant host against cold and freeze stress.
英文关键词	Antarctic soil functional metagenomics freeze-thaw desiccation tolerance Water Hypersensitivity protein late embryogenesis abundant protein
类型	Article
语种	英语
国家	South Africa
收录类别	SCI-E
WOS记录号	WOS:000359823500002
WOS关键词	EMBRYOGENESIS ABUNDANT PROTEIN ; DESICCATION-TOLERANCE ; ARABIDOPSIS-THALIANA ; SIGNAL PEPTIDES ; PREDICTION ; STRESS ; RESPONSES ; IDENTIFICATION ; MEMBRANES ; TOPOLOGY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/187265
作者单位	1.Univ Western Cape, Inst Microbial Biotechnol & Metagen, Dept Biotechnol, ZA-7535 Cape Town, South Africa; 2.Univ Pretoria, Ctr Microbial Ecol & Genom, Dept Genet, ZA-0028 Pretoria, South Africa
推荐引用方式 GB/T 7714	Anderson, Dominique,Ferreras, Eloy,Trindade, Marla,et al. A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage[J],2015,362(15).
APA	Anderson, Dominique,Ferreras, Eloy,Trindade, Marla,&Cowan, Don.(2015).A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage.FEMS MICROBIOLOGY LETTERS,362(15).
MLA	Anderson, Dominique,et al."A novel bacterial Water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage".FEMS MICROBIOLOGY LETTERS 362.15(2015).