Arid
DOI10.1093/femsle/fnv021
Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur
Oren, Aharon1; Mana, Lily1; Jehlicka, Jan2
通讯作者Oren, Aharon
来源期刊FEMS MICROBIOLOGY LETTERS
ISSN0378-1097
EISSN1574-6968
出版年2015
卷号362期号:6
英文摘要

We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells.


英文关键词Allochromatium vinosum Allochromatium warmingii purple sulfur bacteria Raman spectroscopy sulfur carotenoids
类型Article
语种英语
国家Israel ; Czech Republic
收录类别SCI-E
WOS记录号WOS:000356885900009
WOS关键词VIBRATIONAL SPECTROSCOPY ; HALOPHILIC ARCHAEA ; ATACAMA DESERT ; GYPSUM CRUST ; IDENTIFICATION ; THIORHODACEAE ; COMMUNITIES ; SCYTONEMIN ; OXIDATION
WOS类目Microbiology
WOS研究方向Microbiology
来源机构Hebrew University of Jerusalem
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/187263
作者单位1.Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Plant & Environm Sci, IL-91904 Jerusalem, Israel;
2.Charles Univ Prague, Inst Geochem Mineral & Mineral Resources, Prague 12843, Czech Republic
推荐引用方式
GB/T 7714
Oren, Aharon,Mana, Lily,Jehlicka, Jan. Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur[J]. Hebrew University of Jerusalem,2015,362(6).
APA Oren, Aharon,Mana, Lily,&Jehlicka, Jan.(2015).Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur.FEMS MICROBIOLOGY LETTERS,362(6).
MLA Oren, Aharon,et al."Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur".FEMS MICROBIOLOGY LETTERS 362.6(2015).
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