Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1093/femsle/fnv021 |
Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur | |
Oren, Aharon1; Mana, Lily1; Jehlicka, Jan2 | |
通讯作者 | Oren, Aharon |
来源期刊 | FEMS MICROBIOLOGY LETTERS
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ISSN | 0378-1097 |
EISSN | 1574-6968 |
出版年 | 2015 |
卷号 | 362期号:6 |
英文摘要 | We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells. |
英文关键词 | Allochromatium vinosum Allochromatium warmingii purple sulfur bacteria Raman spectroscopy sulfur carotenoids |
类型 | Article |
语种 | 英语 |
国家 | Israel ; Czech Republic |
收录类别 | SCI-E |
WOS记录号 | WOS:000356885900009 |
WOS关键词 | VIBRATIONAL SPECTROSCOPY ; HALOPHILIC ARCHAEA ; ATACAMA DESERT ; GYPSUM CRUST ; IDENTIFICATION ; THIORHODACEAE ; COMMUNITIES ; SCYTONEMIN ; OXIDATION |
WOS类目 | Microbiology |
WOS研究方向 | Microbiology |
来源机构 | Hebrew University of Jerusalem |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/187263 |
作者单位 | 1.Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Plant & Environm Sci, IL-91904 Jerusalem, Israel; 2.Charles Univ Prague, Inst Geochem Mineral & Mineral Resources, Prague 12843, Czech Republic |
推荐引用方式 GB/T 7714 | Oren, Aharon,Mana, Lily,Jehlicka, Jan. Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur[J]. Hebrew University of Jerusalem,2015,362(6). |
APA | Oren, Aharon,Mana, Lily,&Jehlicka, Jan.(2015).Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur.FEMS MICROBIOLOGY LETTERS,362(6). |
MLA | Oren, Aharon,et al."Probing single cells of purple sulfur bacteria with Raman spectroscopy: carotenoids and elemental sulfur".FEMS MICROBIOLOGY LETTERS 362.6(2015). |
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