Arid
DOI10.1007/s00412-014-0499-0
A step to the gigantic genome of the desert locust: chromosome sizes and repeated DNAs
Camacho, J. P. M.1; Ruiz-Ruano, F. J.1; Martin-Blazquez, R.1; Lopez-Leon, M. D.1; Cabrero, J.1; Lorite, P.2; Cabral-de-Mello, D. C.3; Bakkali, M.1
通讯作者Bakkali, M.
来源期刊CHROMOSOMA
ISSN0009-5915
EISSN1432-0886
出版年2015
卷号124期号:2页码:263-275
英文摘要

The desert locust (Schistocerca gregaria) has been used as material for numerous cytogenetic studies. Its genome size is estimated to be 8.55 Gb of DNA comprised in 11 autosomes and the X chromosome. Its X0/XX sex chromosome determinism therefore results in females having 24 chromosomes whereas males have 23. Surprisingly, little is known about the DNA content of this locust’s huge chromosomes. Here, we use the Feulgen Image Analysis Densitometry and C-banding techniques to respectively estimate the DNA quantity and heterochromatin content of each chromosome. We also identify three satellite DNAs using both restriction endonucleases and next-generation sequencing. We then use fluorescent in situ hybridization to determine the chromosomal location of these satellite DNAs as well as that of six tandem repeat DNA gene families. The combination of the results obtained in this work allows distinguishing between the different chromosomes not only by size, but also by the kind of repetitive DNAs that they contain. The recent publication of the draft genome of the migratory locust (Locusta migratoria), the largest animal genome hitherto sequenced, invites for sequencing even larger genomes. S. gregaria is a pest that causes high economic losses. It is thus among the primary candidates for genome sequencing. But this species genome is about 50 % larger than that of L. migratoria, and although next-generation sequencing currently allows sequencing large genomes, sequencing it would mean a greater challenge. The chromosome sizes and markers provided here should not only help planning the sequencing project and guide the assembly but would also facilitate assigning assembled linkage groups to actual chromosomes.


类型Article
语种英语
国家Spain ; Brazil
收录类别SCI-E
WOS记录号WOS:000355146500011
WOS关键词SCHISTOCERCA-GREGARIA FORSKAL ; GRASSHOPPER EYPREPOCNEMIS-PLORANS ; HIGHLY REPEATED DNA ; SATELLITE DNA ; X-RAYS ; CHIASMA DISTRIBUTION ; MALE MEIOSIS ; LAMPBRUSH CHROMOSOMES ; REPETITIVE SEQUENCES ; B-CHROMOSOMES
WOS类目Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/186497
作者单位1.Univ Granada, Fac Ciencias, Dept Genet, E-18071 Granada, Spain;
2.Univ Jaen, Area Genet, Dept Biol Expt, Jaen 23071, Spain;
3.Univ Estadual Paulista, IB, Dept Biol, BR-13506900 Sao Paulo, Brazil
推荐引用方式
GB/T 7714
Camacho, J. P. M.,Ruiz-Ruano, F. J.,Martin-Blazquez, R.,et al. A step to the gigantic genome of the desert locust: chromosome sizes and repeated DNAs[J],2015,124(2):263-275.
APA Camacho, J. P. M..,Ruiz-Ruano, F. J..,Martin-Blazquez, R..,Lopez-Leon, M. D..,Cabrero, J..,...&Bakkali, M..(2015).A step to the gigantic genome of the desert locust: chromosome sizes and repeated DNAs.CHROMOSOMA,124(2),263-275.
MLA Camacho, J. P. M.,et al."A step to the gigantic genome of the desert locust: chromosome sizes and repeated DNAs".CHROMOSOMA 124.2(2015):263-275.
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