Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1093/trstmh/tru085 |
Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran | |
Mirzaei, Asad1,2; Schweynoch, Carola2; Rouhani, Soheila3; Parvizi, Parviz4; Schoenian, Gabriele2 | |
通讯作者 | Mirzaei, Asad |
来源期刊 | TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
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ISSN | 0035-9203 |
EISSN | 1878-3503 |
出版年 | 2014 |
卷号 | 108期号:8页码:502-512 |
英文摘要 | Zoonotic cutaneous leishmaniasis (ZCL) is a polymorphic disease which may show various symptoms. Genetic diversity of the parasite is suggested to be one of the factors influencing the clinical manifestation of the disease. This study used PCR for the detection and identification of leishmanial parasites at the species level and applied a multilocus microsatellite typing approach for investigating the genetic diversity of Leishmania major isolated from captured rodents in two foci of ZCL in Iran: Turkemen Sahara and Fars province. ITS1-rDNA amplification and subsequent RFLP analyses were performed using DNA extracted from the rodents’ ears. Approximately one third of the rodents tested positive for Leishmania; in all rodents L. major was the predominating infecting agent. Seven Rhombomys opimus were positive for L. turanica DNA and one for both L. major and L. turanica. DNA of L. infantum was identified in one Rh. opimus. Seventeen strains of L. major, 15 from Turkemen Sahara and two from Fars province, isolated from different rodents were tested for variation at nine polymorphic microsatellite loci. Ten different MLMT genotypes were observed. They were compared to 89 previously published microsatellite profiles obtained for strains of L. major of different geographical origin. Bayesian model-based and genetic distance based approaches confirmed that strains from Turkemen Sahara and from Fars are genetically different and belong to different genetic groups, largely corresponding to their geographical origins. The considerable genetic variability of L. major might be related to differences in reservoir host and/or to the existence of different populations of the vector, Phlebotomus papatasi. |
英文关键词 | Genetic polymorphism Iran Leishmania major Microsatellite RFLP |
类型 | Article |
语种 | 英语 |
国家 | Iran ; Germany |
收录类别 | SCI-E |
WOS记录号 | WOS:000339954100009 |
WOS关键词 | RESERVOIR ; EPIDEMIOLOGY ; TRANSMISSION ; DIAGNOSIS ; OPIMUS ; HOST ; AREA |
WOS类目 | Public, Environmental & Occupational Health ; Tropical Medicine |
WOS研究方向 | Public, Environmental & Occupational Health ; Tropical Medicine |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/185158 |
作者单位 | 1.Univ Med Sci, Fac Med, Dept Parasitol, Ilam, Iran; 2.Charite, Inst Mikrobiol & Hyg, D-13353 Berlin, Germany; 3.Shahid Beheshti Univ Med Sci, Dept Parasitol, Tehran, Iran; 4.Pasteur Inst Iran, Dept Parasitol, Mol Systemat Lab, Tehran, Iran |
推荐引用方式 GB/T 7714 | Mirzaei, Asad,Schweynoch, Carola,Rouhani, Soheila,et al. Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran[J],2014,108(8):502-512. |
APA | Mirzaei, Asad,Schweynoch, Carola,Rouhani, Soheila,Parvizi, Parviz,&Schoenian, Gabriele.(2014).Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran.TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE,108(8),502-512. |
MLA | Mirzaei, Asad,et al."Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran".TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE 108.8(2014):502-512. |
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