Arid
DOI10.1093/trstmh/tru085
Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran
Mirzaei, Asad1,2; Schweynoch, Carola2; Rouhani, Soheila3; Parvizi, Parviz4; Schoenian, Gabriele2
通讯作者Mirzaei, Asad
来源期刊TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
ISSN0035-9203
EISSN1878-3503
出版年2014
卷号108期号:8页码:502-512
英文摘要

Zoonotic cutaneous leishmaniasis (ZCL) is a polymorphic disease which may show various symptoms. Genetic diversity of the parasite is suggested to be one of the factors influencing the clinical manifestation of the disease.


This study used PCR for the detection and identification of leishmanial parasites at the species level and applied a multilocus microsatellite typing approach for investigating the genetic diversity of Leishmania major isolated from captured rodents in two foci of ZCL in Iran: Turkemen Sahara and Fars province.


ITS1-rDNA amplification and subsequent RFLP analyses were performed using DNA extracted from the rodents’ ears. Approximately one third of the rodents tested positive for Leishmania; in all rodents L. major was the predominating infecting agent. Seven Rhombomys opimus were positive for L. turanica DNA and one for both L. major and L. turanica. DNA of L. infantum was identified in one Rh. opimus. Seventeen strains of L. major, 15 from Turkemen Sahara and two from Fars province, isolated from different rodents were tested for variation at nine polymorphic microsatellite loci. Ten different MLMT genotypes were observed. They were compared to 89 previously published microsatellite profiles obtained for strains of L. major of different geographical origin. Bayesian model-based and genetic distance based approaches confirmed that strains from Turkemen Sahara and from Fars are genetically different and belong to different genetic groups, largely corresponding to their geographical origins.


The considerable genetic variability of L. major might be related to differences in reservoir host and/or to the existence of different populations of the vector, Phlebotomus papatasi.


英文关键词Genetic polymorphism Iran Leishmania major Microsatellite RFLP
类型Article
语种英语
国家Iran ; Germany
收录类别SCI-E
WOS记录号WOS:000339954100009
WOS关键词RESERVOIR ; EPIDEMIOLOGY ; TRANSMISSION ; DIAGNOSIS ; OPIMUS ; HOST ; AREA
WOS类目Public, Environmental & Occupational Health ; Tropical Medicine
WOS研究方向Public, Environmental & Occupational Health ; Tropical Medicine
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/185158
作者单位1.Univ Med Sci, Fac Med, Dept Parasitol, Ilam, Iran;
2.Charite, Inst Mikrobiol & Hyg, D-13353 Berlin, Germany;
3.Shahid Beheshti Univ Med Sci, Dept Parasitol, Tehran, Iran;
4.Pasteur Inst Iran, Dept Parasitol, Mol Systemat Lab, Tehran, Iran
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GB/T 7714
Mirzaei, Asad,Schweynoch, Carola,Rouhani, Soheila,et al. Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran[J],2014,108(8):502-512.
APA Mirzaei, Asad,Schweynoch, Carola,Rouhani, Soheila,Parvizi, Parviz,&Schoenian, Gabriele.(2014).Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran.TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE,108(8),502-512.
MLA Mirzaei, Asad,et al."Diversity of Leishmania species and of strains of Leishmania major isolated from desert rodents in different foci of cutaneous leishmaniasis in Iran".TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE 108.8(2014):502-512.
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