干旱区生态环境知识资源中心(Arid): Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus

Arid

DOI	10.3390/toxins6071979
	Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus
	Barber, Carmel M.1; Madaras, Frank 2; Turnbull, Richard K.3; Morley, Terry 4; Dunstan, Nathan 5; Allen, Luke 5; Kuchel, Tim 3; Mirtschin, Peter 2; Hodgson, Wayne C.1
通讯作者	Hodgson, Wayne C.
来源期刊	TOXINS
ISSN	2072-6651
出版年	2014
卷号	6 期号:7 页码:1979-1995
英文摘要	Taipans are highly venomous Australo-Papuan elapids. A new species of taipan, the Western Desert Taipan (Oxyuranus temporalis), has been discovered with two specimens housed in captivity at the Adelaide Zoo. This study is the first investigation of O. temporalis venom and seeks to characterise and compare the neurotoxicity, lethality and biochemical properties of O. temporalis venom with other taipan venoms. Analysis of O. temporalis venom using size-exclusion and reverse-phase HPLC indicated a markedly simplified "profile" compared to other taipan venoms. SDS-PAGE and agarose gel electrophoresis analysis also indicated a relatively simple composition. Murine LD50 studies showed that O. temporalis venom is less lethal than O. microlepidotus venom. Venoms were tested in vitro, using the chick biventer cervicis nerve-muscle preparation. Based on t(90) values, O. temporalis venom is highly neurotoxic abolishing indirect twitches far more rapidly than other taipan venoms. O. temporalis venom also abolished responses to exogenous acetylcholine and carbachol, indicating the presence of postsynaptic neurotoxins. Prior administration of CSL Taipan antivenom (CSL Limited) neutralised the inhibitory effects of all taipan venoms. The results of this study suggest that the venom of the O. temporalis is highly neurotoxic in vitro and may contain procoagulant toxins, making this snake potentially dangerous to humans.
英文关键词	Oxyuranus temporalis taipan antivenom neurotoxicity snake venom
类型	Article
语种	英语
国家	Australia
收录类别	SCI-E
WOS记录号	WOS:000340039400002
WOS关键词	PHARMACOLOGICAL CHARACTERIZATION ; PRESYNAPTIC NEUROTOXIN ; POSTSYNAPTIC NEUROTOXIN ; S-SCUTELLATUS ; SNAKE-VENOMS ; ANTIVENOM ; MICROLEPIDOTUS ; ACTIVATOR ; EFFICACY
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/185150
作者单位	1.Monash Univ, Dept Pharmacol, Monash Venom Grp, Fac Med Nursing & Hlth Sci, Clayton, Vic 3168, Australia; 2.Venom Sci Pty Ltd, Tanunda, SA 5352, Australia; 3.SA Pathol, IMVS Vet Serv, Gilles Plains, SA 5086, Australia; 4.Adelaide Zoo, Adelaide, SA 5000, Australia; 5.Venom Supplies, Tanunda, SA 5352, Australia
推荐引用方式 GB/T 7714	Barber, Carmel M.,Madaras, Frank,Turnbull, Richard K.,et al. Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus[J],2014,6(7):1979-1995.
APA	Barber, Carmel M..,Madaras, Frank.,Turnbull, Richard K..,Morley, Terry.,Dunstan, Nathan.,...&Hodgson, Wayne C..(2014).Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus.TOXINS,6(7),1979-1995.
MLA	Barber, Carmel M.,et al."Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus".TOXINS 6.7(2014):1979-1995.