Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s00425-013-1981-2 |
Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants | |
Mueller, Werner E. G.1; Qiang, Li1,2; Schroder, Heinz C.1; Honig, Natalie1; Yuan, Daoxian2; Grebenjuk, Vlad A.1; Mussino, Francesca3; Giovine, Marco4; Wang, Xiaohong1 | |
通讯作者 | Mueller, Werner E. G. |
来源期刊 | PLANTA
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ISSN | 0032-0935 |
EISSN | 1432-2048 |
出版年 | 2014 |
卷号 | 239期号:1页码:213-229 |
英文摘要 | Karstification is a rapid process during which calcidic stones/limestones undergo dissolution with the consequence of a desertification of karst regions. A slow-down of those dissolution processes of Ca-carbonate can be approached by a reforestation program using karst-resistant plants that can resist alkaline pH and higher bicarbonate (HCO3 (-)) concentrations in the soil. Carbonic anhydrases (CA) are enzymes that mediate a rapid and reversible interconversion of CO2 and HCO3 (-). In the present study, the steady-state expression of a CA gene, encoding for the plant carbonic anhydrase from the parsley Petroselinum crispum, is monitored. The studies were primarily been performed during germination of the seeds up to the 12/14-day-old embryos. The CA cDNA was cloned. Quantitative polymerase chain reaction (qPCR) analysis revealed that the gene expression level of the P. crispum CA is strongly and significantly affected at more alkaline pH in the growth medium (pH 8.3). This abolishing effect is counteracted both by addition of HCO3 (-) and by addition of polyphosphate (polyP) to the culture medium. In response to polyP, the increased pH in the vacuoles of the growing plants is normalized. The effect of polyP let us to propose that this polymer acts as a buffer system that facilitates the adjustment of the pH in the cytoplasm. In addition, it is proposed that polyP has the potential to act, especially in the karst, as a fertilizer that allows the karstic plants to cope with the adverse pH and HCO3 (-) condition in the soil. |
英文关键词 | Bicarbonate Carbonic anhydrase Fertilizer Gene expression Intracellular pH Karst Parsley Petroselinum Polyphosphate Vacuoles |
类型 | Article |
语种 | 英语 |
国家 | Germany ; Peoples R China ; Italy |
收录类别 | SCI-E |
WOS记录号 | WOS:000329240700016 |
WOS关键词 | VACUOLAR PH ; SUBERITES-DOMUNCULA ; GENE-EXPRESSION ; CELLS ; BICARBONATE ; ACCUMULATION ; TEMPERATURE ; ANTISENSE ; REDUCTION ; EVOLUTION |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/184299 |
作者单位 | 1.Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Univ Med Ctr, ERC Adv Investigator Grant Res Grp, D-55128 Mainz, Germany; 2.Inst Karst Geol, CAGS, Guilin, Guangxi, Peoples R China; 3.Univ Genoa, Dept Expt Med, Biochem Sect, I-16132 Genoa, Italy; 4.Univ Genoa, DISTAV, I-16132 Genoa, Italy |
推荐引用方式 GB/T 7714 | Mueller, Werner E. G.,Qiang, Li,Schroder, Heinz C.,et al. Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants[J],2014,239(1):213-229. |
APA | Mueller, Werner E. G..,Qiang, Li.,Schroder, Heinz C..,Honig, Natalie.,Yuan, Daoxian.,...&Wang, Xiaohong.(2014).Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants.PLANTA,239(1),213-229. |
MLA | Mueller, Werner E. G.,et al."Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants".PLANTA 239.1(2014):213-229. |
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