Arid
DOI10.1007/s00425-013-1981-2
Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants
Mueller, Werner E. G.1; Qiang, Li1,2; Schroder, Heinz C.1; Honig, Natalie1; Yuan, Daoxian2; Grebenjuk, Vlad A.1; Mussino, Francesca3; Giovine, Marco4; Wang, Xiaohong1
通讯作者Mueller, Werner E. G.
来源期刊PLANTA
ISSN0032-0935
EISSN1432-2048
出版年2014
卷号239期号:1页码:213-229
英文摘要

Karstification is a rapid process during which calcidic stones/limestones undergo dissolution with the consequence of a desertification of karst regions. A slow-down of those dissolution processes of Ca-carbonate can be approached by a reforestation program using karst-resistant plants that can resist alkaline pH and higher bicarbonate (HCO3 (-)) concentrations in the soil. Carbonic anhydrases (CA) are enzymes that mediate a rapid and reversible interconversion of CO2 and HCO3 (-). In the present study, the steady-state expression of a CA gene, encoding for the plant carbonic anhydrase from the parsley Petroselinum crispum, is monitored. The studies were primarily been performed during germination of the seeds up to the 12/14-day-old embryos. The CA cDNA was cloned. Quantitative polymerase chain reaction (qPCR) analysis revealed that the gene expression level of the P. crispum CA is strongly and significantly affected at more alkaline pH in the growth medium (pH 8.3). This abolishing effect is counteracted both by addition of HCO3 (-) and by addition of polyphosphate (polyP) to the culture medium. In response to polyP, the increased pH in the vacuoles of the growing plants is normalized. The effect of polyP let us to propose that this polymer acts as a buffer system that facilitates the adjustment of the pH in the cytoplasm. In addition, it is proposed that polyP has the potential to act, especially in the karst, as a fertilizer that allows the karstic plants to cope with the adverse pH and HCO3 (-) condition in the soil.


英文关键词Bicarbonate Carbonic anhydrase Fertilizer Gene expression Intracellular pH Karst Parsley Petroselinum Polyphosphate Vacuoles
类型Article
语种英语
国家Germany ; Peoples R China ; Italy
收录类别SCI-E
WOS记录号WOS:000329240700016
WOS关键词VACUOLAR PH ; SUBERITES-DOMUNCULA ; GENE-EXPRESSION ; CELLS ; BICARBONATE ; ACCUMULATION ; TEMPERATURE ; ANTISENSE ; REDUCTION ; EVOLUTION
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/184299
作者单位1.Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Univ Med Ctr, ERC Adv Investigator Grant Res Grp, D-55128 Mainz, Germany;
2.Inst Karst Geol, CAGS, Guilin, Guangxi, Peoples R China;
3.Univ Genoa, Dept Expt Med, Biochem Sect, I-16132 Genoa, Italy;
4.Univ Genoa, DISTAV, I-16132 Genoa, Italy
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GB/T 7714
Mueller, Werner E. G.,Qiang, Li,Schroder, Heinz C.,et al. Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants[J],2014,239(1):213-229.
APA Mueller, Werner E. G..,Qiang, Li.,Schroder, Heinz C..,Honig, Natalie.,Yuan, Daoxian.,...&Wang, Xiaohong.(2014).Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants.PLANTA,239(1),213-229.
MLA Mueller, Werner E. G.,et al."Carbonic anhydrase: a key regulatory and detoxifying enzyme for Karst plants".PLANTA 239.1(2014):213-229.
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