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The taxa of the Leucophyllum pringlei clade were used to understand the influence of the Neogene orogenesis and the Quaternary climate cycles on the diversification of the flora of the xeric regions of North America. This clade includes the five southernmost species of the genus: L. ambiguum, L. flyrii, L. pruinosum and L ultramonticola, which are distributed throughout the Chihuahuan Desert north of the Trans-Mexican Volcanic Belt, and L pringlei in the region of Tehuacan-Cuicatlan south of this mountain range. Here we test whether these species diverged during the pluvial periods of the Pleistocene, and whether L. pringlei diverged earlier from the other species during the uplift of the Trans-Mexican Volcanic Belt. Using three plastid regions (psbA-trnH, psbK-psbI, trnL-F) and a nuclear (ITS) marker, phylogenetic analyses were carried out, along with a reconstruction of their ancestral area. Trees retrieved the five species in a monophyletic group with the most recent common ancestor distributed in the Sinaloan dry forest during the Late Miocene (8.08 Ma), from where it dispersed to the Chihuahuan Desert during the Late Miocene (6.35 Ma). The secondary uplift of the Sierra Madre Occidental during the Late Miocene to Early Pliocene influenced a vicariance event. Divergence between L. pringlei and the species from north of the Trans-Mexican Volcanic Belt occurred during the second volcanic episode in the Late Miocene (7.5-3 Ma). The most recent common ancestor of L ambiguum, L pruinosum and L. ultramonticola was widely distributed in the southern part of the Chihuahuan Desert during the Early to Late Pliocene (3.50 Ma). The diversification of these three species occurred in the Middle Pleistocene (0.9 Ma) during the pluvial and inter-pluvial cycles. (C) 2014 Elsevier Inc. All rights reserved.