Arid
DOI10.1007/s13353-013-0176-7
Buffalo alpha S1-casein gene 5 ’-flanking region and its interspecies comparison
Patel, Amrutlal K.1; Singh, Mahavir2; Suryanarayana, V. V. S.3,4
通讯作者Singh, Mahavir
来源期刊JOURNAL OF APPLIED GENETICS
ISSN1234-1983
EISSN2190-3883
出版年2014
卷号55期号:1页码:75-87
英文摘要

The expression of milk protein genes is tightly regulated in a spatio-temporal manner through the combinatorial interaction of lactogenic hormones and a set of transcription factors mediating developmental and tissue-specific gene expression. The recruitment of a unique set of transcription factors is determined by the cis-regulatory motifs present in the gene promoter region. Here, we report the isolation, sequencing, structural analysis and interspecies comparison of the 5’cis-regulatory region of the buffalo alpha S1 (alpha S1)-casein gene. The proximal promoter region of the buffalo alpha S1-casein gene harbored the insertion of a 72-bp fragment of long interspersed nuclear element of the L1_BT retrotransposon family. Among the core and vertebrate-specific promoter elements, the motifs for the binding of Brn POU domain factors (BRNF), Lim homeodomain factors (LHXF), NK6 homeobox transcription factors (NKX6), nuclear factor kappa B/c-rel (NFKB), AT-rich interactive domain factor (ARID), Brn POU domain factor 5 (BRN5), pancreatic and intestinal homeodomain transcription factor (PDX1), Distal-less homeodomain transcription factors (DLXF), T-cell factor/lymphoid enhancer-binding factor-1 (LEFF) and GHF-1 pituitary-specific POU domain transcription factor (PIT1) were over-represented in the alpha S1-casein gene regulatory region (Z score > 4.0). The Multiple EM for Motif elicitation predicted three motifs which consisted of the sequences known to bind mammary gland factor/signal transducer and activator of transcription 5 (MGF/STAT5), estrogen receptor-related alpha (ER alpha), steroidogenic factor 1 (SF1) and glucocorticoid receptor (GR), indicating their potential role in the mammary gland-specific gene expression. The interspecies comparison of the proximal promoter region revealed conserved sequences for TATA boxes and MGF/STAT5 in all species, whereas activator protein 1 (AP1), pregnancy-specific mammary nuclear factor (PMF), CCAAT/enhancer binding protein (C/EBP), double-stranded and single-stranded DNA-binding protein 1 (DS1 and SS), ying and yang factor 1 (YY1), and GR half-sites were among ruminants. The functional significance of the L1_BT retrotransposon insertion on the buffalo alpha S1-casein gene expression needs to be experimentally validated.


英文关键词Milk proteins alpha S1-casein gene Murrah buffalo cis-regulatory motifs Repetitive elements
类型Article
语种英语
国家India ; USA
收录类别SCI-E
WOS记录号WOS:000331197900007
WOS关键词MAMMARY EPITHELIAL-CELLS ; BETA-CASEIN PROMOTER ; PLASMINOGEN-ACTIVATOR CDNA ; GLAND FACTOR ; DNA-BINDING ; GLUCOCORTICOID-RECEPTOR ; ALPHA-S1-CASEIN GENE ; STRUCTURAL FEATURES ; FLANKING REGION ; FACTOR MGF
WOS类目Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/182937
作者单位1.Anand Agr Univ, Coll Vet Sci & Anim Husb, Dept Anim Biotechnol, Anand, Gujarat, India;
2.Univ Louisville, Sch Med, IMC, Diabet & Obes Ctr, Louisville, KY 40202 USA;
3.Indian Vet Res Inst, Dept Vet Biotechnol, Izatnagar 243122, Uttar Pradesh, India;
4.Indian Vet Res Inst, Mol Virol Lab, Bangalore 560024, Karnataka, India
推荐引用方式
GB/T 7714
Patel, Amrutlal K.,Singh, Mahavir,Suryanarayana, V. V. S.. Buffalo alpha S1-casein gene 5 ’-flanking region and its interspecies comparison[J],2014,55(1):75-87.
APA Patel, Amrutlal K.,Singh, Mahavir,&Suryanarayana, V. V. S..(2014).Buffalo alpha S1-casein gene 5 ’-flanking region and its interspecies comparison.JOURNAL OF APPLIED GENETICS,55(1),75-87.
MLA Patel, Amrutlal K.,et al."Buffalo alpha S1-casein gene 5 ’-flanking region and its interspecies comparison".JOURNAL OF APPLIED GENETICS 55.1(2014):75-87.
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