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Holocene hydrological changes in regions dominated by the westerlies significantly differ from those by the Asian summer monsoon. The high-elevated northeastern Tibetan Plateau, located in between, is likely influenced by the interactions of both circulation systems. Here, we attempt to use biomarkers, n-alkanes and alkenones, to reconstruct Holocene lake-level changes at Lake Gahai in the Qaidam Basin. We choose a sediment core drilled at the lake shore, where biomarkers would be sensitive to lake-level changes. The n-alkane records show high average chain length (ACL), high carbon preference index (CPI), and low proportion of aquatic macrophyte (P-aq) values at 7-2 kyr (thousand calibrated years ago) with peaked values around 6 kyr, whereas low ACL, low CPI, and high P-aq values occurred after 2 kyr and before 7 kyr. No alkenones were detected at 7-2 kyr, suggesting that lake level at this period was incapable of constantly reaching to the coring site. Therefore, combined results provide unambiguous evidence of relatively low lake level at 7-2 kyr, probably lowest at similar to 6 kyr. Holocene lake-level changes in this marginal region thus display a different pattern from either of the core regions dominated by the westerlies (anti-phase) and the Asian summer monsoon (out-of-phase). We suggest that in the arid marginal region, temperature-induced evaporation could significantly affect regional hydrological balance, resulting in the discrepancy with the Holocene long-term precipitation decreasing trend in Asian monsoon-dominated regions.