Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.gene.2014.01.013 |
Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco | |
Deng, Long-Qun1; Yu, Hao-Qiang1; Liu, Yan-Ping2; Jiao, Pei-Pei2; Zhou, Shu-Feng1; Zhang, Su-Zhi1; Li, Wan-Chen1; Fu, Feng-Ling1 | |
通讯作者 | Li, Wan-Chen |
来源期刊 | GENE |
ISSN | 0378-1119 |
EISSN | 1879-0038 |
出版年 | 2014 |
卷号 | 539期号:1页码:132-140 |
英文摘要 | Antifreeze proteins are a class of polypeptides produced by certain animals, plants, fungi and bacteria that permit their survival under the subzero environments. Ammopiptanthus nanus is the unique evergreen broadleaf bush endemic to the Mid-Asia deserts. It survives at the west edge of the Tarim Basin from the disappearance of the ancient Mediterranean in the Tertiary Period. Its distribution region is characterized by the arid climate and extreme temperatures, where the extreme temperatures range from - 30 degrees C to 40 degrees C. In the present study, the antifreeze protein gene AnAFP of A. nanus was used to transform Escherichia coli and tobacco, after bioinformatics analysis for its possible function. The transformed E. coli strain expressed the heterologous AnAFP gene under the induction of isopropyl beta-D-thiogalactopyranoside, and demonstrated significant enhancement of cold tolerance. The transformed tobacco lines expressed the heterologous AnAFP gene in response to cold stress, and showed a less change of relative electrical conductivity under cold stress, and a less wilting phenotype after 16 h of 3 C cold stress and thawing for 1 h than the untransformed wild-type plants. All these results imply the potential value of the AnAFP gene to be used in genetic modification of commercially important crops for improvement of cold tolerance. (C) 2014 Elsevier B.V. All rights reserved. |
英文关键词 | Ammopiptanthus nanus Antifreeze protein Cold tolerance Heterologous expression Escherichia coli Tobacco |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000333496500019 |
WOS关键词 | THERMAL HYSTERESIS PROTEINS ; RICH-REPEAT PROTEIN ; WINTER RYE LEAVES ; TRANSGENIC TOBACCO ; ICE RECRYSTALLIZATION ; LOLIUM-PERENNE ; TARGETED EXPRESSION ; PHYSICAL-PROPERTIES ; ANTARCTIC FISHES ; SYNTHETIC GENE |
WOS类目 | Genetics & Heredity |
WOS研究方向 | Genetics & Heredity |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/182182 |
作者单位 | 1.Sichuan Agr Univ, Maize Res Inst, Chengdu 611130, Sichuan, Peoples R China; 2.Tarim Univ, Fac Plant Sci, Alar 843300, Xinjiang, Peoples R China |
推荐引用方式 GB/T 7714 | Deng, Long-Qun,Yu, Hao-Qiang,Liu, Yan-Ping,et al. Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco[J],2014,539(1):132-140. |
APA | Deng, Long-Qun.,Yu, Hao-Qiang.,Liu, Yan-Ping.,Jiao, Pei-Pei.,Zhou, Shu-Feng.,...&Fu, Feng-Ling.(2014).Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco.GENE,539(1),132-140. |
MLA | Deng, Long-Qun,et al."Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco".GENE 539.1(2014):132-140. |
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