Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.foodres.2014.03.013 |
Antioxidant activity and characterization of constituents in copao fruits (Eulychnia acida Phil., Cactaceae) by HPLC-DAD-MS/MSn | |
Jimenez-Aspee, Felipe1; Quispe, Cristina1; Soriano, Maria Del Pilar C.1; Gonzalez, Jaime Fuentes1; Haneke, Eva1; Theoduloz, Cristina2; Schmeda-Hirschmann, Guillermo1 | |
通讯作者 | Schmeda-Hirschmann, Guillermo |
来源期刊 | FOOD RESEARCH INTERNATIONAL
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ISSN | 0963-9969 |
EISSN | 1873-7145 |
出版年 | 2014 |
卷号 | 62页码:286-298 |
英文摘要 | Copao (Eulychnia acida Phil., Cactaceae) is an endemic species occurring in arid areas of northern Chile. The fruits are commercialized by peasants within the Elqui and Limari valleys and are appreciated for its acidic and refreshing taste. We now report the total phenolic (TP) and total flavonoid (TF) content, antioxidant activity, phenolic composition and main phenolic distribution in pulp and epicarp of copao fruits from different harvesting places from both valleys. The ascorbic acid content was determined in fresh fruit pulp, epicarp and juice. The phenolic-enriched extract was analyzed for antioxidant effect and composition. Ferulic acid, 9,10-dihydroxy-4,7-megastigmadien-3-one hexoside, isorhamnetin and quercetin glycosides were identified by HPLC-DAD-MS/MS analysis. The main compounds were isolated and fully characterized by NMR techniques. The main phenolic in the samples was isorhamnetin-3-O-[alpha-rhamnopyranosyl-(1 -> 6)-beta-glucopyranoside]. The HPLC pattern of the phenolic-enriched extracts of the fruits allows a differentiation of samples from the Elqui and Limari valleys. All fruit extracts and Amberlite-retained fraction from the methanolic extract were devoid of toxicity against human gastric AGS cells and human lung fibroblasts, with IC50 values >400 mu g/mL for AGS and 344 to >400 mu g/mL for fibroblasts, respectively. The compound identification, associated with the antioxidant activity and insignificant cell toxicity, adds relevant information for the possible development of this native fruit into a new crop. (C) 2014 Elsevier Ltd. All rights reserved. |
英文关键词 | Eulychnia acida Cactaceae Copao Phenolics Antioxidants Arid lands |
类型 | Article |
语种 | 英语 |
国家 | Chile |
收录类别 | SCI-E |
WOS记录号 | WOS:000340015100035 |
WOS关键词 | LIQUID-CHROMATOGRAPHY ; PHENOLIC-COMPOUNDS ; CHEMICAL-CHARACTERIZATION ; ESI-MS ; OPUNTIA ; L. ; QUANTIFICATION ; IDENTIFICATION ; CAPACITY ; BETALAIN |
WOS类目 | Food Science & Technology |
WOS研究方向 | Food Science & Technology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/182094 |
作者单位 | 1.Univ Talca, Inst Quim Recursos Nat, Lab Quim Prod Nat, Talca 3460000, Chile; 2.Univ Talca, Fac Ciencias Salud, Lab Cultivo Celular, Talca 3460000, Chile |
推荐引用方式 GB/T 7714 | Jimenez-Aspee, Felipe,Quispe, Cristina,Soriano, Maria Del Pilar C.,et al. Antioxidant activity and characterization of constituents in copao fruits (Eulychnia acida Phil., Cactaceae) by HPLC-DAD-MS/MSn[J],2014,62:286-298. |
APA | Jimenez-Aspee, Felipe.,Quispe, Cristina.,Soriano, Maria Del Pilar C..,Gonzalez, Jaime Fuentes.,Haneke, Eva.,...&Schmeda-Hirschmann, Guillermo.(2014).Antioxidant activity and characterization of constituents in copao fruits (Eulychnia acida Phil., Cactaceae) by HPLC-DAD-MS/MSn.FOOD RESEARCH INTERNATIONAL,62,286-298. |
MLA | Jimenez-Aspee, Felipe,et al."Antioxidant activity and characterization of constituents in copao fruits (Eulychnia acida Phil., Cactaceae) by HPLC-DAD-MS/MSn".FOOD RESEARCH INTERNATIONAL 62(2014):286-298. |
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