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DOI10.1007/s12224-013-9153-5
How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas
Chlumska, Zuzana1,2; Janecek, Stepan2; Dolezal, Jiri1,2
通讯作者Chlumska, Zuzana
来源期刊FOLIA GEOBOTANICA
ISSN1211-9520
EISSN1874-9348
出版年2014
卷号49期号:1页码:1-15
英文摘要

Nonstructural carbohydrates (NSC) are frequently studied both by ecologists and plant physiologists because they serve as fundamental carbon and energy sources in plant metabolism. Because of the rapid enzymatic hydrolysis of NSC after harvest, plants are usually placed into liquid nitrogen until subsequent analyses in the laboratory. Nevertheless, when the research is performed in poorly accessible regions such as high mountains, tropical forests or deserts, the use of heavy containers containing vaporizing liquid nitrogen is problematic for logistical reasons. These places are particularly interesting as they harbor plant species with interesting physiological adaptations. In our study we aimed to develop a suitable storage method for plants intended for NSC analyses, which would require minimal equipment. These demands resulted in the idea of using the first step in NSC analyses - extraction in ethanol. Samples were extracted in 50 % and 96 % boiling or non-boiling ethanol and then stored for one month; they were compared with samples immediately frozen in liquid nitrogen. We discovered that for samples containing starch, fructans, soluble sugars and sugar alcohols, the best pretreatment for subsequent storage is extraction in 50 % or 96 % boiling ethanol. For total nonstructural carbohydrates (TNC) assessment, only extracting with 50 % ethanol without boiling gave very good results. Finally, we developed a method that could be used in any remote place without bulky laboratory equipment.


英文关键词Ethanol extraction Liquid nitrogen Nonstructural carbohydrates Plant tissue storage
类型Article
语种英语
国家Czech Republic
收录类别SCI-E
WOS记录号WOS:000334419400001
WOS关键词PHRAGMITES-AUSTRALIS ; CHEMICAL-CHANGES ; SALT STRESS ; GROWTH ; STORAGE ; SUCROSE ; LEAVES ; METABOLISM ; SEEDLINGS ; RESERVES
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/182077
作者单位1.Univ South Bohemia, Fac Sci, Dept Bot, CZ-37005 Ceske Budejovice, Czech Republic;
2.Acad Sci Czech Republic, Inst Bot, Dept Funct Ecol, CZ-37982 Trebon, Czech Republic
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GB/T 7714
Chlumska, Zuzana,Janecek, Stepan,Dolezal, Jiri. How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas[J],2014,49(1):1-15.
APA Chlumska, Zuzana,Janecek, Stepan,&Dolezal, Jiri.(2014).How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas.FOLIA GEOBOTANICA,49(1),1-15.
MLA Chlumska, Zuzana,et al."How to Preserve Plant Samples for Carbohydrate Analysis? Test of Suitable Methods Applicable in Remote Areas".FOLIA GEOBOTANICA 49.1(2014):1-15.
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