干旱区生态环境知识资源中心

A high performance liquid chromatography-tandem mass spectrometric method (HPLC-MS/MS) was developed for the simultaneous determination of 31 kinds of triazine herbicides in farmland soils. Samples were extracted by accelerated solvent extraction (ASE) and purified by Oasis MCX solid phase extraction (SPE) cartridges, and then analyzed by HPLC-MS/MS in multiple reaction monitoring (MRM) mode with positive electrospray ionization. The analytical column was Phenomenex Luna C-18 (150 mm x 2.0 mm x 3 mu m) and the mobile phases were acetonitrile and water containing 0.1% (V/V) formic acid. The limits of detection (S/N >= 3) were 0.008-0.440 mu g/L. All of the triazine herbicides had good linear responses with r >= 0.996 and the average recoveries in the spiked levels of 0.40-40.0 mu g/kg ranged from 76.9%-102.0% with the RSDs of 3.4%-10.3%. The HPLC-MS/MS method had been applied for the detection of the triazine herbicide residues in farmland soils from Shenyang region and the results showed that atrazine, simazine, prometryne and atrazine-desethyl were the main triazine herbicides in the region.