Arid
DOI10.1186/s12870-014-0312-5
Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium
Yurchenko, Olga P.1; Park, Sunjung1,2; Ilut, Daniel C.3; Inmon, Jay J.1; Millhollon, Jon C.1; Liechty, Zach4; Page, Justin T.4; Jenks, Matthew A.5; Chapman, Kent D.2; Udall, Joshua A.4; Gore, Michael A.3; Dyer, John M.1
通讯作者Dyer, John M.
来源期刊BMC PLANT BIOLOGY
ISSN1471-2229
出版年2014
卷号14
英文摘要

Background: The majority of commercial cotton varieties planted worldwide are derived from Gossypium hirsutum, which is a naturally occurring allotetraploid produced by interspecific hybridization of A-and D-genome diploid progenitor species. While most cotton species are adapted to warm, semi-arid tropical and subtropical regions, and thus perform well in these geographical areas, cotton seedlings are sensitive to cold temperature, which can significantly reduce crop yields. One of the common biochemical responses of plants to cold temperatures is an increase in omega-3 fatty acids, which protects cellular function by maintaining membrane integrity. The purpose of our study was to identify and characterize the omega-3 fatty acid desaturase (FAD) gene family in G. hirsutum, with an emphasis on identifying omega-3 FADs involved in cold temperature adaptation.


Results: Eleven omega-3 FAD genes were identified in G. hirsutum, and characterization of the gene family in extant A and D diploid species (G. herbaceum and G. raimondii, respectively) allowed for unambiguous genome assignment of all homoeologs in tetraploid G. hirsutum. The omega-3 FAD family of cotton includes five distinct genes, two of which encode endoplasmic reticulum-type enzymes (FAD3-1 and FAD3-2) and three that encode chloroplast-type enzymes (FAD7/8-1, FAD7/8-2, and FAD7/8-3). The FAD3-2 gene was duplicated in the A genome progenitor species after the evolutionary split from the D progenitor, but before the interspecific hybridization event that gave rise to modern tetraploid cotton. RNA-seq analysis revealed conserved, gene-specific expression patterns in various organs and cell types and semi-quantitative RT-PCR further revealed that FAD7/8-1 was specifically induced during cold temperature treatment of G. hirsutum seedlings.


Conclusions: The omega-3 FAD gene family in cotton was characterized at the genome-wide level in three species, showing relatively ancient establishment of the gene family prior to the split of A and D diploid progenitor species. The FAD genes are differentially expressed in various organs and cell types, including fiber, and expression of the FAD7/8-1 gene was induced by cold temperature. Collectively, these data define the genetic and functional genomic properties of this important gene family in cotton and provide a foundation for future efforts to improve cotton abiotic stress tolerance through molecular breeding approaches.


英文关键词Chilling tolerance Cotton Drought Fatty acid desaturase Gossypium Linolenic acid Omega-3 fatty acid
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000346931100007
WOS关键词FATTY-ACID DESATURATION ; MULTIPLE SEQUENCE ALIGNMENT ; TRANSGENIC TOBACCO ; COTTON SEEDLINGS ; LOW-TEMPERATURE ; COLD TOLERANCE ; ALLOPOLYPLOID COTTON ; ARABIDOPSIS-THALIANA ; MAXIMUM-LIKELIHOOD ; OMEGA-3 DESATURASE
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/181178
作者单位1.USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA;
2.Univ N Texas, Dept Biol Sci, Ctr Plant Lipid Res, Denton, TX 76203 USA;
3.Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA;
4.Brigham Young Univ, Plant & Wildlife Sci Dept, Provo, UT 84602 USA;
5.W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA
推荐引用方式
GB/T 7714
Yurchenko, Olga P.,Park, Sunjung,Ilut, Daniel C.,et al. Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium[J],2014,14.
APA Yurchenko, Olga P..,Park, Sunjung.,Ilut, Daniel C..,Inmon, Jay J..,Millhollon, Jon C..,...&Dyer, John M..(2014).Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium.BMC PLANT BIOLOGY,14.
MLA Yurchenko, Olga P.,et al."Genome-wide analysis of the omega-3 fatty acid desaturase gene family in Gossypium".BMC PLANT BIOLOGY 14(2014).
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