Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1186/1471-2229-14-44 |
EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobacco | |
Li, Xiaoshuang1,2,3; Zhang, Daoyuan1; Li, Haiyan1,2; Wang, Yucheng1; Zhang, Yuanming1![]() | |
通讯作者 | Zhang, Daoyuan |
来源期刊 | BMC PLANT BIOLOGY
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ISSN | 1471-2229 |
出版年 | 2014 |
卷号 | 14 |
英文摘要 | Background: Dehydration-Responsive Element-Binding Protein2 (DREB2) is a transcriptional factor which regulates the expression of several stress-inducible genes. DREB2-type proteins are particularly important in plant responses to drought, salt and heat. DREB2 genes have been identified and characterized in a variety of plants, and DREB2 genes are promising candidate genes for the improvement of stress tolerance in plants. However, little is known about these genes in plants adapted to water-limiting environments. Results: In this study, we describe the characterization of EsDREB2B, a novel DREB2B gene identified from the desert plant Eremosparton songoricum. Phylogenetic analysis and motif prediction indicate that EsDREB2B encodes a truncated DREB2 polypeptide that belongs to a legume-specific DREB2 group. In E. songoricum, EsDREB2B transcript accumulation was induced by a variety of abiotic stresses, including drought, salinity, cold, heat, heavy metal, mechanical wounding, oxidative stress and exogenous abscisic acid (ABA) treatment. Consistent with the predicted role as a transcription factor, EsDREB2B was targeted to the nucleus of onion epidermal cells and exhibited transactivation activity of a GAL4-containing reporter gene in yeast. In transgenic yeast, overexpression of EsDREB2B increased tolerance to multiple abiotic stresses. Our findings indicate that EsDREB2B can enhance stress tolerance in other plant species. Heterologous expression of EsDREB2B in tobacco showed improved tolerance to multiple abiotic stresses, and the transgenic plants exhibited no reduction in foliar growth. We observed that EsDREB2B is a functional DREB2-orthologue able to influence the physiological and biochemical response of transgenic tobacco to stress. Conclusions: Based upon these findings, EsDREB2B encodes an abiotic stress-inducible, transcription factor which confers abiotic stress-tolerance in yeast and transgenic tobacco. |
英文关键词 | Cold DREB Drought Heat Proline Salt Stress Transcript accumulation Transgenic tobacco |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000334473600001 |
WOS关键词 | RESPONSIVE GENE-EXPRESSION ; FUNCTIONAL-CHARACTERIZATION ; WATER-STRESS ; SACCHAROMYCES-CEREVISIAE ; REGULATORY NETWORKS ; DROUGHT TOLERANCE ; BINDING-PROTEIN ; ARABIDOPSIS ; PLANTS ; DEHYDRATION |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
来源机构 | 中国科学院新疆生态与地理研究所 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/181176 |
作者单位 | 1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Xinjiang 830011, Urumqi, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA |
推荐引用方式 GB/T 7714 | Li, Xiaoshuang,Zhang, Daoyuan,Li, Haiyan,et al. EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobacco[J]. 中国科学院新疆生态与地理研究所,2014,14. |
APA | Li, Xiaoshuang,Zhang, Daoyuan,Li, Haiyan,Wang, Yucheng,Zhang, Yuanming,&Wood, Andrew J..(2014).EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobacco.BMC PLANT BIOLOGY,14. |
MLA | Li, Xiaoshuang,et al."EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobacco".BMC PLANT BIOLOGY 14(2014). |
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