Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1186/1471-2229-14-2 |
The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment | |
Yates, Steven A.1; Chernukhin, Igor1; Alvarez-Fernandez, Ruben1; Bechtold, Ulrike1; Baeshen, Mohammed2; Baeshen, Nabih2; Mutwakil, Mohammad Z.2; Sabir, Jamal2; Lawson, Tracy1; Mullineaux, Philip M.1 | |
通讯作者 | Mullineaux, Philip M. |
来源期刊 | BMC PLANT BIOLOGY
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ISSN | 1471-2229 |
出版年 | 2014 |
卷号 | 14 |
英文摘要 | Background: The perennial species Rhazya stricta (R. stricta) grows in arid zones and carries out typical C-3 photosynthesis under daily extremes of heat, light intensity and low humidity. In order to identify processes attributable to its adaptation to this harsh environment, we profiled the foliar transcriptome of apical and mature leaves harvested from the field at three time periods of the same day. Results: Next generation sequencing was used to reconstruct the transcriptome and quantify gene expression. 28018 full length transcript sequences were recovered and 45.4% were differentially expressed (DE) throughout the day. We compared our dataset with microarray experiments in Arabidopsis thaliana (Arabidopsis) and other desert species to identify trends in circadian and stress response profiles between species. 34% of the DE genes were homologous to Arabidopsis circadian-regulated genes. Independent of circadian control, significant overlaps with Arabidopsis genes were observed only with heat and salinity/high light stress-responsive genes. Also, groups of DE genes common to other desert plants species were identified. We identified protein families specific to R. stricta which were found to have diverged from their homologs in other species and which were over -expressed at midday. Conclusions: This study shows that temporal profiling is essential to assess the significance of genes apparently responsive to abiotic stress. This revealed that in R. stricta, the circadian clock is a major regulator of DE genes, even of those annotated as stress-responsive in other species. This may be an important feature of the adaptation of R. stricta to its extreme but predictable environment. However, the majority of DE genes were not circadian-regulated. Of these, some were common to other desert species and others were distinct to R. stricta, suggesting that they are important for the adaptation of such plants to arid environments. |
英文关键词 | Next generation sequencing Transcriptomics Circadian clock Rhazya stricta Perennial desert plants Heat stress Salinity stress C-3 photosynthesis |
类型 | Article |
语种 | 英语 |
国家 | England ; Saudi Arabia |
收录类别 | SCI-E |
WOS记录号 | WOS:000334469000001 |
WOS关键词 | ARABIDOPSIS CIRCADIAN CLOCK ; ABSCISIC-ACID BIOSYNTHESIS ; DUPLEX-SPECIFIC NUCLEASE ; HEAT-SHOCK PROTEINS ; RNA-SEQ ; SIGNAL-TRANSDUCTION ; STRESS TOLERANCE ; INDUCIBLE GENES ; SALICYLIC-ACID ; ABIOTIC STRESS |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/181175 |
作者单位 | 1.Univ Essex, Sch Biol Sci, Colchester CO4 3SQ, Essex, England; 2.King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah 21589, Saudi Arabia |
推荐引用方式 GB/T 7714 | Yates, Steven A.,Chernukhin, Igor,Alvarez-Fernandez, Ruben,et al. The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment[J],2014,14. |
APA | Yates, Steven A..,Chernukhin, Igor.,Alvarez-Fernandez, Ruben.,Bechtold, Ulrike.,Baeshen, Mohammed.,...&Mullineaux, Philip M..(2014).The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment.BMC PLANT BIOLOGY,14. |
MLA | Yates, Steven A.,et al."The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment".BMC PLANT BIOLOGY 14(2014). |
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