Arid
DOI10.1186/1471-2229-14-2
The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment
Yates, Steven A.1; Chernukhin, Igor1; Alvarez-Fernandez, Ruben1; Bechtold, Ulrike1; Baeshen, Mohammed2; Baeshen, Nabih2; Mutwakil, Mohammad Z.2; Sabir, Jamal2; Lawson, Tracy1; Mullineaux, Philip M.1
通讯作者Mullineaux, Philip M.
来源期刊BMC PLANT BIOLOGY
ISSN1471-2229
出版年2014
卷号14
英文摘要

Background: The perennial species Rhazya stricta (R. stricta) grows in arid zones and carries out typical C-3 photosynthesis under daily extremes of heat, light intensity and low humidity. In order to identify processes attributable to its adaptation to this harsh environment, we profiled the foliar transcriptome of apical and mature leaves harvested from the field at three time periods of the same day.


Results: Next generation sequencing was used to reconstruct the transcriptome and quantify gene expression. 28018 full length transcript sequences were recovered and 45.4% were differentially expressed (DE) throughout the day. We compared our dataset with microarray experiments in Arabidopsis thaliana (Arabidopsis) and other desert species to identify trends in circadian and stress response profiles between species. 34% of the DE genes were homologous to Arabidopsis circadian-regulated genes. Independent of circadian control, significant overlaps with Arabidopsis genes were observed only with heat and salinity/high light stress-responsive genes. Also, groups of DE genes common to other desert plants species were identified. We identified protein families specific to R. stricta which were found to have diverged from their homologs in other species and which were over -expressed at midday.


Conclusions: This study shows that temporal profiling is essential to assess the significance of genes apparently responsive to abiotic stress. This revealed that in R. stricta, the circadian clock is a major regulator of DE genes, even of those annotated as stress-responsive in other species. This may be an important feature of the adaptation of R. stricta to its extreme but predictable environment. However, the majority of DE genes were not circadian-regulated. Of these, some were common to other desert species and others were distinct to R. stricta, suggesting that they are important for the adaptation of such plants to arid environments.


英文关键词Next generation sequencing Transcriptomics Circadian clock Rhazya stricta Perennial desert plants Heat stress Salinity stress C-3 photosynthesis
类型Article
语种英语
国家England ; Saudi Arabia
收录类别SCI-E
WOS记录号WOS:000334469000001
WOS关键词ARABIDOPSIS CIRCADIAN CLOCK ; ABSCISIC-ACID BIOSYNTHESIS ; DUPLEX-SPECIFIC NUCLEASE ; HEAT-SHOCK PROTEINS ; RNA-SEQ ; SIGNAL-TRANSDUCTION ; STRESS TOLERANCE ; INDUCIBLE GENES ; SALICYLIC-ACID ; ABIOTIC STRESS
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/181175
作者单位1.Univ Essex, Sch Biol Sci, Colchester CO4 3SQ, Essex, England;
2.King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah 21589, Saudi Arabia
推荐引用方式
GB/T 7714
Yates, Steven A.,Chernukhin, Igor,Alvarez-Fernandez, Ruben,et al. The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment[J],2014,14.
APA Yates, Steven A..,Chernukhin, Igor.,Alvarez-Fernandez, Ruben.,Bechtold, Ulrike.,Baeshen, Mohammed.,...&Mullineaux, Philip M..(2014).The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment.BMC PLANT BIOLOGY,14.
MLA Yates, Steven A.,et al."The temporal foliar transcriptome of the perennial C-3 desert plant Rhazya stricta in its natural environment".BMC PLANT BIOLOGY 14(2014).
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