Arid
DOI10.1186/1471-2164-15-1061
RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes
McGivern, James J.1; Wray, Kenneth P.1; Margres, Mark J.1; Couch, Michelle E.1; Mackessy, Stephen P.2; Rokyta, Darin R.1
通讯作者Rokyta, Darin R.
来源期刊BMC GENOMICS
ISSN1471-2164
出版年2014
卷号15
英文摘要

Background: Largely because of their direct, negative impacts on human health, the venoms of front-fanged snakes of the families Viperidae and Elapidae have been extensively characterized proteomically, transcriptomically, and pharmacologically. However, relatively little is known about the molecular complexity and evolution of the venoms of rear-fanged colubrid snakes, which are, with a few notable exceptions, regarded as harmless to humans. Many of these snakes have venoms with major effects on their preferred prey, and their venoms are probably as critical to their survival as those of front-fanged elapids and viperids.


Results: We sequenced the venom-gland transcriptomes from a specimen of Hypsiglena (Desert Night Snake; family Colubridae, subfamily Dipsadinae) and of Boiga irregularis (Brown Treesnake; family Colubridae, subfamily Colubrinae) and verified the transcriptomic results proteomically by means of high-definition mass spectrometry. We identified nearly 3,000 nontoxin genes for each species. For B. irregularis, we found 108 putative toxin transcripts in 46 clusters with < 1% nucleotide divergence, and for Hypsiglena we identified 79 toxin sequences that were grouped into 33 clusters. Comparisons of the venoms revealed divergent venom types, with Hypsiglena possessing a viper-like venom dominated by metalloproteinases, and B. irregularis having a more elapid-like venom, consisting primarily of three-finger toxins.


Conclusions: Despite the difficulty of procuring venom from rear-fanged species, we were able to complete all analyses from a single specimen of each species without pooling venom samples or glands, demonstrating the power of high-definition transcriptomic and proteomic approaches. We found a high level of divergence in the venom types of two colubrids. These two venoms reflected the hemorrhagic/neurotoxic venom dichotomy that broadly characterizes the difference in venom strategies between elapids and viperids.


类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000347569100001
WOS关键词LECTIN-LIKE PROTEINS ; DIAMONDBACK RATTLESNAKE ; PHYLOGENETIC ANALYSIS ; GLAND TRANSCRIPTOME ; 3-FINGER TOXIN ; ADAPTIVE EVOLUTION ; BOIGA-IRREGULARIS ; FAMILY ; IDENTIFICATION ; PROTEOME
WOS类目Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/181172
作者单位1.Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA;
2.Univ No Colorado, Sch Biol Sci, Greeley, CO 80639 USA
推荐引用方式
GB/T 7714
McGivern, James J.,Wray, Kenneth P.,Margres, Mark J.,et al. RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes[J],2014,15.
APA McGivern, James J.,Wray, Kenneth P.,Margres, Mark J.,Couch, Michelle E.,Mackessy, Stephen P.,&Rokyta, Darin R..(2014).RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes.BMC GENOMICS,15.
MLA McGivern, James J.,et al."RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes".BMC GENOMICS 15(2014).
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