Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1186/1471-2164-15-1061 |
RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes | |
McGivern, James J.1; Wray, Kenneth P.1; Margres, Mark J.1; Couch, Michelle E.1; Mackessy, Stephen P.2; Rokyta, Darin R.1 | |
通讯作者 | Rokyta, Darin R. |
来源期刊 | BMC GENOMICS
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ISSN | 1471-2164 |
出版年 | 2014 |
卷号 | 15 |
英文摘要 | Background: Largely because of their direct, negative impacts on human health, the venoms of front-fanged snakes of the families Viperidae and Elapidae have been extensively characterized proteomically, transcriptomically, and pharmacologically. However, relatively little is known about the molecular complexity and evolution of the venoms of rear-fanged colubrid snakes, which are, with a few notable exceptions, regarded as harmless to humans. Many of these snakes have venoms with major effects on their preferred prey, and their venoms are probably as critical to their survival as those of front-fanged elapids and viperids. Results: We sequenced the venom-gland transcriptomes from a specimen of Hypsiglena (Desert Night Snake; family Colubridae, subfamily Dipsadinae) and of Boiga irregularis (Brown Treesnake; family Colubridae, subfamily Colubrinae) and verified the transcriptomic results proteomically by means of high-definition mass spectrometry. We identified nearly 3,000 nontoxin genes for each species. For B. irregularis, we found 108 putative toxin transcripts in 46 clusters with < 1% nucleotide divergence, and for Hypsiglena we identified 79 toxin sequences that were grouped into 33 clusters. Comparisons of the venoms revealed divergent venom types, with Hypsiglena possessing a viper-like venom dominated by metalloproteinases, and B. irregularis having a more elapid-like venom, consisting primarily of three-finger toxins. Conclusions: Despite the difficulty of procuring venom from rear-fanged species, we were able to complete all analyses from a single specimen of each species without pooling venom samples or glands, demonstrating the power of high-definition transcriptomic and proteomic approaches. We found a high level of divergence in the venom types of two colubrids. These two venoms reflected the hemorrhagic/neurotoxic venom dichotomy that broadly characterizes the difference in venom strategies between elapids and viperids. |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000347569100001 |
WOS关键词 | LECTIN-LIKE PROTEINS ; DIAMONDBACK RATTLESNAKE ; PHYLOGENETIC ANALYSIS ; GLAND TRANSCRIPTOME ; 3-FINGER TOXIN ; ADAPTIVE EVOLUTION ; BOIGA-IRREGULARIS ; FAMILY ; IDENTIFICATION ; PROTEOME |
WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/181172 |
作者单位 | 1.Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA; 2.Univ No Colorado, Sch Biol Sci, Greeley, CO 80639 USA |
推荐引用方式 GB/T 7714 | McGivern, James J.,Wray, Kenneth P.,Margres, Mark J.,et al. RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes[J],2014,15. |
APA | McGivern, James J.,Wray, Kenneth P.,Margres, Mark J.,Couch, Michelle E.,Mackessy, Stephen P.,&Rokyta, Darin R..(2014).RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes.BMC GENOMICS,15. |
MLA | McGivern, James J.,et al."RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes".BMC GENOMICS 15(2014). |
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