干旱区生态环境知识资源中心(Arid): Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library

Arid

DOI	10.1007/s10529-013-1403-3
	Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library
	Zhang, Qian 1; Xu, Hui 2; Zhao, Jing 2; Zeng, Runying 2
通讯作者	Zeng, Runying
来源期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
EISSN	1573-6776
出版年	2014
卷号	36 期号:3 页码:617-625
英文摘要	One clone (ACPGA001) exhibiting penicillin G acylase (PGA) activity was screened from a metagenomic library by using a medium containing penicillin G. A novel PGA gene from the inserted fragment of ACPGA001 was obtained by sequencing. The amino acid sequence of ACPGA001 PGA exhibited < 33 % similarity to PGAs retrieved from GenBank. This gene was expressed in Escherichia coli M15 and the recombinant protein was purified and characterized. The ACPGA001 PGA exhibited a maximum activity at 60 A degrees C and showed high activity at pH 4-10 with an optimum pH of 8.0. This enzyme was stable at 40 A degrees C for 70 min with a half-life of 60 min at 55 A degrees C. These beneficial characteristics of ACPGA001 PGA provide some advantages for the potential application of ACPGA001 PGA in industry.
英文关键词	Characterization Gene expression Metagenomic library Penicillin G acylase
类型	Article
语种	英语
国家	USA ; Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000333349300025
WOS关键词	ANTARCTIC DESERT SOIL ; MOLECULAR-CLONING ; ARTHROBACTER-VISCOSUS ; BACILLUS-MEGATERIUM ; ESCHERICHIA-COLI ; GENE ; PURIFICATION ; ESTERASE ; SEDIMENT
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/181148
作者单位	1.Univ Hawaii Manoa, Dept Civil & Environm Engn, Honolulu, HI 96822 USA; 2.SOA, Inst Oceanog 3, State Key Lab Breeding Base, Key Lab Marine Genet Resource, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qian,Xu, Hui,Zhao, Jing,et al. Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library[J],2014,36(3):617-625.
APA	Zhang, Qian,Xu, Hui,Zhao, Jing,&Zeng, Runying.(2014).Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library.BIOTECHNOLOGY LETTERS,36(3),617-625.
MLA	Zhang, Qian,et al."Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library".BIOTECHNOLOGY LETTERS 36.3(2014):617-625.