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DOI | 10.1080/09291016.2013.869438 |
Thermal dependency of electrical excitability of gastrocnemius (skeletal) muscle in the desert-reptile, Uromastix hardwickii | |
Soomro, Mohammad Saleh1; Mahar, Saeed Ahmed2; Qureshi, Aijaz Ahmed3; Soomro, Mohammad Naeem4; Soomro, Mohammad Waseem5 | |
通讯作者 | Soomro, Mohammad Saleh |
来源期刊 | BIOLOGICAL RHYTHM RESEARCH
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ISSN | 0929-1016 |
EISSN | 1744-4179 |
出版年 | 2014 |
卷号 | 45期号:4页码:515-522 |
英文摘要 | This study deals with effects of seasonal temperature variations on muscular electrical excitability in the reptile Uromastix hardwickii. Freshly captured adult animals of both sexes were used, and the gastrocnemius (skeletal) muscles were dissected out. The muscle samples were digested with digestive fluid (pepsin and HCl), stirred, and the cells were allowed to settle out. The supernatant was removed leaving a whitish fluid containing clear cells that were used for "patch-clamp" recording of membrane ionic currents and potentials. Resting membrane potentials (RMPs) and action potentials of the cell membranes were measured using the whole-cell current mode. Glass microelectrodes, with a tip diameter 2-3 mu m and tip resistance 5-6 MO (when filled with intracellular solution), were used. The mean values for RMP action potential and its duration showed no significant changes with the season, but other components of action potential, including threshold potential, after-potential, and its duration, were found to increase significantly (p < 0.05) in summer as compared to winter. This is the first study of seasonal temperature dependency of these parameters in the gastrocnemius muscle of U. hardwickii. This study shows that seasonal changes in components of the action potential are associated with changes in environmental temperature. These changes may be responsible for altered activities and homeostasis in these animals, and possibly related to the underlying mechanisms responsible for hibernation. |
英文关键词 | Uromastix hardwickii gastrocnemius muscle environmental temperature patch-clamp technique phases of action potential |
类型 | Article |
语种 | 英语 |
国家 | Pakistan |
收录类别 | SCI-E |
WOS记录号 | WOS:000337580100003 |
WOS关键词 | PATCH-CLAMP ; TISSUE ; GANGLION ; CURRENTS ; NEURONS |
WOS类目 | Biology ; Physiology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/181110 |
作者单位 | 1.Liaquat Natl Med Coll, Dept Physiol, Karachi, Pakistan; 2.Liaquat Natl Hosp & Med Coll, Dept Med, Karachi, Pakistan; 3.Sindh Med Univ, Dept Physiol, Karachi, Pakistan; 4.NED Univ, Civil Dept, Karachi, Pakistan; 5.NED Univ, Dept Mech, Karachi, Pakistan |
推荐引用方式 GB/T 7714 | Soomro, Mohammad Saleh,Mahar, Saeed Ahmed,Qureshi, Aijaz Ahmed,et al. Thermal dependency of electrical excitability of gastrocnemius (skeletal) muscle in the desert-reptile, Uromastix hardwickii[J],2014,45(4):515-522. |
APA | Soomro, Mohammad Saleh,Mahar, Saeed Ahmed,Qureshi, Aijaz Ahmed,Soomro, Mohammad Naeem,&Soomro, Mohammad Waseem.(2014).Thermal dependency of electrical excitability of gastrocnemius (skeletal) muscle in the desert-reptile, Uromastix hardwickii.BIOLOGICAL RHYTHM RESEARCH,45(4),515-522. |
MLA | Soomro, Mohammad Saleh,et al."Thermal dependency of electrical excitability of gastrocnemius (skeletal) muscle in the desert-reptile, Uromastix hardwickii".BIOLOGICAL RHYTHM RESEARCH 45.4(2014):515-522. |
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