Arid
DOI10.1080/21564574.2014.893927
Characterisation of microsatellite markers in the Spotted Sand Lizard (Pedioplanis lineoocellata) shows low levels of inbreeding and moderate genetic diversity on a small spatial scale
Tolley, Krystal A.1,2; Daniels, Ryan J.1,2; Feldheim, Kevin A.3
通讯作者Tolley, Krystal A.
来源期刊AFRICAN JOURNAL OF HERPETOLOGY
ISSN2156-4574
EISSN2153-3660
出版年2014
卷号63期号:2页码:87-97
英文摘要

Population genetic methods can be useful for understanding spatial genetic patterns, gene flow and diversity. While genetic markers such as gene sequences are useful for understanding broad scale phylogeographic patterns, microsatellite markers allow for inferences within species and on smaller spatial scales. Such data can then be used to glean information on biological processes at the landscape level. Because portions of southern Africa are predicted to be heavily impacted by climatic changes forecast for the next century, a better understanding of the biota at the species level would be beneficial for advancing knowledge on the southern African system. Therefore, we characterised 11 microsatellite markers for an arid adapted lacertid lizard, Pedioplanis lineoocellata, in order to obtain a microsatellite library for future studies. Five of 11 loci were out of Hardy-Weinberg (H-W) equilibrium, showing a homozygote excess. Frequency-based analysis suggested this is due to the presence of null alleles. We further investigated this using a model-based Bayesian approach to simultaneously estimate inbreeding, null alleles and random amplification failures. In contrast to the frequency-based approach, this method indicated that both inbreeding and random amplification failures, not null alleles, are the most significant components of the model for nine of 11 loci. Overall, the inbreeding coefficient was low, but not zero, suggesting that low levels of inbreeding contribute to H-W deviations. Finally, we examined whether population bottlenecks could be a factor in deviations from H-W using multiple methods, but found no evidence to suggest that bottlenecks have played a role.


英文关键词Africa inbreeding Lacertidae null alleles population bottlenecks population genetics reptiles
类型Article
语种英语
国家South Africa ; USA
收录类别SCI-E
WOS记录号WOS:000344392200001
WOS关键词RECENT POPULATION BOTTLENECKS ; ALLELE FREQUENCY DATA ; NULL ALLELES ; CLIMATE-CHANGE ; LOCI ; DNA ; RESPONSES ; COEFFICIENTS ; LACERTIDAE ; DISPERSAL
WOS类目Zoology
WOS研究方向Zoology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/180469
作者单位1.South African Natl Biodivers Inst, Appl Biodivers Res Div, Claremont, South Africa;
2.Univ Stellenbosch, Dept Bot & Zool, Matieland, South Africa;
3.Field Museum, Pritzker Lab Mol Systemat & Evolut, Chicago, IL USA
推荐引用方式
GB/T 7714
Tolley, Krystal A.,Daniels, Ryan J.,Feldheim, Kevin A.. Characterisation of microsatellite markers in the Spotted Sand Lizard (Pedioplanis lineoocellata) shows low levels of inbreeding and moderate genetic diversity on a small spatial scale[J],2014,63(2):87-97.
APA Tolley, Krystal A.,Daniels, Ryan J.,&Feldheim, Kevin A..(2014).Characterisation of microsatellite markers in the Spotted Sand Lizard (Pedioplanis lineoocellata) shows low levels of inbreeding and moderate genetic diversity on a small spatial scale.AFRICAN JOURNAL OF HERPETOLOGY,63(2),87-97.
MLA Tolley, Krystal A.,et al."Characterisation of microsatellite markers in the Spotted Sand Lizard (Pedioplanis lineoocellata) shows low levels of inbreeding and moderate genetic diversity on a small spatial scale".AFRICAN JOURNAL OF HERPETOLOGY 63.2(2014):87-97.
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