Arid
DOI10.1007/s11738-014-1486-z
An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers
Phulwaria, Mahendra1; Patel, Ashok Kumar1; Rathore, Jitendra Singh1,2; Ram, Kheta1; Shekhawat, N. S.1
通讯作者Phulwaria, Mahendra
来源期刊ACTA PHYSIOLOGIAE PLANTARUM
ISSN0137-5881
EISSN1861-1664
出版年2014
卷号36期号:5页码:1115-1122
英文摘要

An improved micropropagation method has been developed for Salvadora oleoides, a valuable tree species of alkaline and arid regions. Nodal explant obtained from a mature tree (30- to 35-year-old) responded optimally (80.0 %) on BAP (2.0 mg l(-1)) and produced (4.56 +/- A 0.52) shoots. Shoots were further multiplied by subculturing the in vitro excised shoots and transferring them to MS medium containing either BAP (0.0-2.0 mg l(-1)) alone or in combination with lower concentrations of an auxin (IAA or NAA 0.05-0.4 mg l(-1)). Among all the PGRs combination tested, MS medium supplemented with BAP (0.5 mg l(-1)) and IAA (0.1 mg l(-1)) formed the maximum number of shoots (68.40 +/- A 2.74 per culture bottle) with an average height (6.59 +/- A 0.30 cm), after 6 weeks of culture. Rooting in regenerated shoots was achieved by ex vitro methods and about 92.5 % of shoots were rooted with 5.25 +/- A 0.64 roots per shoot and an average length of 2.76 +/- A 0.53 cm after 3 weeks of incubation in the green house. More than (80 %) of hardened plantlets survived in the field conditions. Genetic stability of the discussed protocol was confirmed by two DNA-based fingerprinting techniques i.e. RAPD and ISSR. Of the 10 RAPD primers finally selected, a total of 42 bands (out of 43) were monomorphic and one polymorphic, whereas from 10 ISSR primers selected, all the 43 bands were monomorphic revealing a high level of genetic homogeneity in the regenerated plants and the donor plant. In the present investigation, we achieved significantly more number of shoots during multiplication, which are higher than all previous reports and further evaluated the genetic fidelity of protocol for the first time in S. oleoides, which concludes the clonal (true-to-type) nature of micropropagated plantlets.


英文关键词DNA-based markers Ex vitro rooting Genetic homogeneity S. oleoides
类型Article
语种英语
国家India
收录类别SCI-E
WOS记录号WOS:000334442000006
WOS关键词IN-VITRO ; FIDELITY ; TREE ; PLANTS ; L. ; PROPAGATION ; GROWTH
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/180368
作者单位1.Jai Narain Vyas Univ, Dept Bot, Biotechnol Unit, Jodhpur 342001, Rajasthan, India;
2.Lachoo Mem Coll Sci & Technol Autonomous, Jodhpur 342001, Rajasthan, India
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GB/T 7714
Phulwaria, Mahendra,Patel, Ashok Kumar,Rathore, Jitendra Singh,et al. An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers[J],2014,36(5):1115-1122.
APA Phulwaria, Mahendra,Patel, Ashok Kumar,Rathore, Jitendra Singh,Ram, Kheta,&Shekhawat, N. S..(2014).An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers.ACTA PHYSIOLOGIAE PLANTARUM,36(5),1115-1122.
MLA Phulwaria, Mahendra,et al."An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers".ACTA PHYSIOLOGIAE PLANTARUM 36.5(2014):1115-1122.
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