Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1186/1477-5956-11-3 |
Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation | |
Dedieu, Alain1; Sahinovic, Elodie1; Guerin, Philippe1; Blanchard, Laurence2,3,4; Fochesato, Sylvain2,3,4; Meunier, Bruno5; de Groot, Arjan2,3,4; Armengaud, Jean1 | |
通讯作者 | Dedieu, Alain |
来源期刊 | PROTEOME SCIENCE
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EISSN | 1477-5956 |
出版年 | 2013 |
卷号 | 11 |
英文摘要 | Background: Deinococcus deserti VCD115 has been isolated from Sahara surface sand. This radiotolerant bacterium represents an experimental model of choice to understand adaptation to harsh conditions encountered in hot arid deserts. We analysed the soluble proteome dynamics in this environmentally relevant model after exposure to 3 kGy gamma radiation, a non-lethal dose that generates massive DNA damages. For this, cells were harvested at different time lapses after irradiation and their soluble proteome contents have been analysed by 2-DE and mass spectrometry. Results: In the first stage of the time course we observed accumulation of DNA damage response protein DdrB (that shows the highest fold change similar to 11), SSB, and two different RecA proteins (RecA(P) and RecA(C)). Induction of DNA repair protein PprA, DNA damage response protein DdrD and the two gyrase subunits (GyrA and GyrB) was also detected. A response regulator of the SarP family, a type II site-specific deoxyribonuclease and a putative N-acetyltransferase are three new proteins found to be induced. In a more delayed stage, we observed accumulation of several proteins related to central metabolism and protein turn-over, as well as helicase UvrD and novel forms of both gyrase subunits differing in terms of isoelectric point and molecular weight. Conclusions: Post-translational modifications of GyrA (N-terminal methionine removal and acetylation) have been evidenced and their significance discussed. We found that the Deide_02842 restriction enzyme, which is specifically found in D. deserti, is a new potential member of the radiation/desiccation response regulon, highlighting the specificities of D. deserti compared to the D. radiodurans model. |
英文关键词 | Proteome Post-translational modification Irradiation Early response Reference 2D map Kinetics Hierarchical clustering |
类型 | Article |
语种 | 英语 |
国家 | France |
收录类别 | SCI-E |
WOS记录号 | WOS:000314513400001 |
WOS关键词 | STRAND BREAK REPAIR ; IONIZING-RADIATION ; EXTREME RADIORESISTANCE ; TERMINAL ACETYLATION ; DDRB PROTEIN ; DNA-REPAIR ; RADIODURANS ; RECA ; PHOSPHORYLATION ; RESISTANCE |
WOS类目 | Biochemical Research Methods |
WOS研究方向 | Biochemistry & Molecular Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/179520 |
作者单位 | 1.CEA Marcoule, DSV, IBEB, SBTN,LBSP, F-30207 Bagnols Sur Ceze, France; 2.CEA, DSV, IBEB, Lab Ecol Microb Rhizosphere & Environ Extrem LEMi, F-13108 St Paul Les Durance, France; 3.CNRS, UMR 6191, F-13108 St Paul Les Durance, France; 4.Aix Marseille Univ, F-13108 St Paul Les Durance, France; 5.INRA, Herbivores UR1213, F-63122 St Genes Champanelle, France |
推荐引用方式 GB/T 7714 | Dedieu, Alain,Sahinovic, Elodie,Guerin, Philippe,et al. Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation[J],2013,11. |
APA | Dedieu, Alain.,Sahinovic, Elodie.,Guerin, Philippe.,Blanchard, Laurence.,Fochesato, Sylvain.,...&Armengaud, Jean.(2013).Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation.PROTEOME SCIENCE,11. |
MLA | Dedieu, Alain,et al."Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation".PROTEOME SCIENCE 11(2013). |
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