Arid
DOI10.1186/1477-5956-11-3
Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation
Dedieu, Alain1; Sahinovic, Elodie1; Guerin, Philippe1; Blanchard, Laurence2,3,4; Fochesato, Sylvain2,3,4; Meunier, Bruno5; de Groot, Arjan2,3,4; Armengaud, Jean1
通讯作者Dedieu, Alain
来源期刊PROTEOME SCIENCE
EISSN1477-5956
出版年2013
卷号11
英文摘要

Background: Deinococcus deserti VCD115 has been isolated from Sahara surface sand. This radiotolerant bacterium represents an experimental model of choice to understand adaptation to harsh conditions encountered in hot arid deserts. We analysed the soluble proteome dynamics in this environmentally relevant model after exposure to 3 kGy gamma radiation, a non-lethal dose that generates massive DNA damages. For this, cells were harvested at different time lapses after irradiation and their soluble proteome contents have been analysed by 2-DE and mass spectrometry.


Results: In the first stage of the time course we observed accumulation of DNA damage response protein DdrB (that shows the highest fold change similar to 11), SSB, and two different RecA proteins (RecA(P) and RecA(C)). Induction of DNA repair protein PprA, DNA damage response protein DdrD and the two gyrase subunits (GyrA and GyrB) was also detected. A response regulator of the SarP family, a type II site-specific deoxyribonuclease and a putative N-acetyltransferase are three new proteins found to be induced. In a more delayed stage, we observed accumulation of several proteins related to central metabolism and protein turn-over, as well as helicase UvrD and novel forms of both gyrase subunits differing in terms of isoelectric point and molecular weight.


Conclusions: Post-translational modifications of GyrA (N-terminal methionine removal and acetylation) have been evidenced and their significance discussed. We found that the Deide_02842 restriction enzyme, which is specifically found in D. deserti, is a new potential member of the radiation/desiccation response regulon, highlighting the specificities of D. deserti compared to the D. radiodurans model.


英文关键词Proteome Post-translational modification Irradiation Early response Reference 2D map Kinetics Hierarchical clustering
类型Article
语种英语
国家France
收录类别SCI-E
WOS记录号WOS:000314513400001
WOS关键词STRAND BREAK REPAIR ; IONIZING-RADIATION ; EXTREME RADIORESISTANCE ; TERMINAL ACETYLATION ; DDRB PROTEIN ; DNA-REPAIR ; RADIODURANS ; RECA ; PHOSPHORYLATION ; RESISTANCE
WOS类目Biochemical Research Methods
WOS研究方向Biochemistry & Molecular Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/179520
作者单位1.CEA Marcoule, DSV, IBEB, SBTN,LBSP, F-30207 Bagnols Sur Ceze, France;
2.CEA, DSV, IBEB, Lab Ecol Microb Rhizosphere & Environ Extrem LEMi, F-13108 St Paul Les Durance, France;
3.CNRS, UMR 6191, F-13108 St Paul Les Durance, France;
4.Aix Marseille Univ, F-13108 St Paul Les Durance, France;
5.INRA, Herbivores UR1213, F-63122 St Genes Champanelle, France
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GB/T 7714
Dedieu, Alain,Sahinovic, Elodie,Guerin, Philippe,et al. Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation[J],2013,11.
APA Dedieu, Alain.,Sahinovic, Elodie.,Guerin, Philippe.,Blanchard, Laurence.,Fochesato, Sylvain.,...&Armengaud, Jean.(2013).Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation.PROTEOME SCIENCE,11.
MLA Dedieu, Alain,et al."Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation".PROTEOME SCIENCE 11(2013).
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