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In concurrent with global warming, precipitation regimes are predicted to change as well around the world. In this study, two experimental sites were selected with different nutrient availability along a slope to study the effects of simulated increased precipitation on soil P-glucosidase activities in an Inner Mongolian grassland. Soil samples were adjusted to 55% of water holding capacity and incubated at 22 C in the dark for 32 days. Soil beta-glucosidase activities were measured prior to and after the incubation. Results showed that soil beta-glucosidase activities had differential responses to increased precipitation with a significant increase in the down-slope site, but not in the upslope site. Correlation analysis showed that the initial soil beta-glucosidase activities exerted a significantly negative relationship with soluble organic nitrogen (N). Our results indicated that both water availability and soil soluble N availability played important roles in regulating beta-glucosidase activities in this semi-arid region.