Arid
DOI10.1371/journal.pone.0065249
Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing
Shen, Fangxia; Yao, Maosheng
通讯作者Yao, Maosheng
来源期刊PLOS ONE
ISSN1932-6203
出版年2013
卷号8期号:6
英文摘要

In this study, the bacterial and fungal abundances, diversities, conductance levels as well as total organic carbon (TOC) were investigated in the snow samples collected from five different snow occurrences in Beijing between January and March, 2010. The collected snow samples were melted and cultured at three different temperatures (4, 26 and 37 degrees C). The culturable bacterial concentrations were manually counted and the resulting colony forming units (CFUs) at 26 degrees C were further studied using V3 region of 16 S rRNA gene-targeted polymerase chain reaction -denaturing gradient gel electrophoresis (PCR-DGGE). The clone library was constructed after the liquid culturing of snow samples at 26 degrees C. And microscopic method was employed to investigate the fungal diversity in the samples. In addition, outdoor air samples were also collected using mixed cellulose ester (MCE) filters and compared with snow samples with respect to described characteristics. The results revealed that snow samples had bacterial concentrations as much as 16000 CFU/ml for those cultured at 26 degrees C, and the conductance levels ranged from 5.6x10(-6) to 2.4x10(-5) S. PCR-DGGE, sequencing and microscopic analysis revealed remarkable bacterial and fungal diversity differences between the snow samples and the outdoor air samples. In addition, DGGE banding profiles for the snow samples collected were also shown distinctly different from one another. Absent from the outdoor air, certain human, plant, and insect fungal pathogens were found in the snow samples. By calculation, culturable bacteria accounted for an average of 3.38% (+/- 1.96%) of TOC for the snow samples, and 0.01% for that of outdoor air samples. The results here suggest that snow precipitations are important sources of fungal pathogens and ice nucleators, thus could affect local climate, human health and agriculture security.


类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000321099000048
WOS关键词MICROBIAL COMMUNITY STRUCTURE ; SACCHAROMYCES-CEREVISIAE ; AIRBORNE MICROORGANISMS ; SIZE DISTRIBUTION ; FUNGAL SPORES ; MOUTH-DISEASE ; DESERT DUST ; BACTERIA ; AEROSOL ; CULTURABILITY
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
来源机构北京大学
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/179373
作者单位Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shen, Fangxia,Yao, Maosheng. Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing[J]. 北京大学,2013,8(6).
APA Shen, Fangxia,&Yao, Maosheng.(2013).Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing.PLOS ONE,8(6).
MLA Shen, Fangxia,et al."Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing".PLOS ONE 8.6(2013).
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