干旱区生态环境知识资源中心(Arid): Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry

Arid

DOI	10.1021/jf4001296
	Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry
	Zhao, Liang 1,2; Meng, Kun 1; Bai, Yingguo 1; Shi, Pengjun 1; Huang, Huoqing 1; Luo, Huiying 1; Wang, Yaru 1; Yang, Peilong 1; Song, Wei 2; Yao, Bin 1
通讯作者	Yao, Bin
来源期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
EISSN	1520-5118
出版年	2013
卷号	61 期号:28 页码:6880-6889
英文摘要	This study identified two family-11 xylanase genes (xynC81 and xynC83) in Achaetomium sp. Xz-8, a thermophilic strain from a desert area with substantial xylanase activity, and successfully expressed them in Pichia pastoris. Their deduced amino acid sequences showed the highest identity of <= 90% to known fungal xylanases and of <= 62% with each other. The purified recombinant xylanases showed optimal activities at pH 5.5 and 60-65 degrees C and exhibited stability over pH 5.0-10.0 and temperatures at 55 degrees C and below. XynC81 had high catalytic efficiency (6082 mL/s/mg), and XynC83 was favorable for xylooligosaccharide production. Under simulated mashing conditions, combination of XynC83 and a commercial beta-glucanase improved the filtration rate by 34.7696, which is much better than that of Novozymes Ultraflo (20.71%). XynC81 and XynC83 had a synergistic effect on viscosity reduction (7.08%), which is comparable with that of Ultraflo (8.47%). Thus, XynC81 and XynC83 represent good candidates for application in the brewing industry.
英文关键词	Achaetomium xylanase heterologous expression catalytic efficiency brewing
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000322103600015
WOS关键词	CARBOHYDRATE-BINDING MODULES ; MOLECULAR-CLONING ; PICHIA-PASTORIS ; EXPRESSION ; GENE ; FUNGI ; HEMICELLULASES ; HYDROLASES ; BARLEY ; ENZYME
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/177892
作者单位	1.Chinese Acad Agr Sci, Key Lab Feed Biotechnol, Minist Agr, Feed Res Inst, Beijing 100081, Peoples R China; 2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Liang,Meng, Kun,Bai, Yingguo,et al. Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry[J],2013,61(28):6880-6889.
APA	Zhao, Liang.,Meng, Kun.,Bai, Yingguo.,Shi, Pengjun.,Huang, Huoqing.,...&Yao, Bin.(2013).Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,61(28),6880-6889.
MLA	Zhao, Liang,et al."Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.28(2013):6880-6889.