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The Shoushan Basin is an important hydrocarbon province in the northern Western Desert, Egypt, but the burial/thermal histories for most of the source rocks in the basin have not been assigned yet. In this study, subsurface samples from selected wells were collected to characterize the source rocks of Alam El-Bueib Formation and to study thermal history in the Shoushan Basin. The Lower Cretaceous Alam El-Bueib Formation is widespread in the Shoushan Basin, which is composed mainly of shales and sandstones with minor carbonate rocks deposited in a marine environment. The gas generative potential of the Lower Cretaceous Alam El-Bueib Formation in the Shoushan Basin was evaluated by Rock-Eval pyrolysis. Most samples contain sufficient type III organic matter to be considered gas prone. Vitrinite reflectance was measured at eight stratigraphic levels (Jurassic-Cretaceous). Vitrinite reflectance profiles show a general increase of vitrinite reflectance with depth. Vitrinite reflectance values of Alam El-Bueib Formation range between 0.70 and 0.87 VRr %, indicating a thermal maturity level sufficient for hydrocarbon generation. Thermal maturity and burial histories models predict that the Alam El-Bueib source rock entered the mid-mature stage for hydrocarbon generation in the Tertiary. These models indicate that the onset of gas generation from the Alam El-Bueib source rock began in the Paleocene (60 Ma), and the maximum volume of gas generation occurred during the Pliocene (3-2 Ma).