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DOI | 10.1016/j.ibmb.2012.10.002 |
Regulation of feeding by Neuropeptide F in the desert locust, Schistocerca gregaria | |
Van Wielendaele, Pieter; Dillen, Senne; Zels, Sven; Badisco, Liesbeth; Vanden Broeck, Jozef | |
通讯作者 | Van Wielendaele, Pieter |
来源期刊 | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
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ISSN | 0965-1748 |
EISSN | 1879-0240 |
出版年 | 2013 |
卷号 | 43期号:1页码:102-114 |
英文摘要 | Our knowledge on the physiological function of the insect Neuropeptide F (NPF) mostly comes from studies in the fruit fly, Drosophila melanogaster, where NPF was shown to regulate diverse processes, such as feeding, learning and responding to stress. In the desert locust, Schistocerca gregaria, only a truncated form of the "full-length" NPF (the biologically active "trNPF’) has been isolated. In this study, we investigated whether this peptide is involved in the regulation of feeding in this orthopteran species. In the S. gregaria EST-database, an NPF-precursor encoding transcript was found. Alignment with other insect NPF-precursors showed relatively highest sequence conservation within the trNPF region (and the flanking dibasic cleavage site), as compared to other regions of the NPF-precursor. Quantitative real-time RT-PCR revealed that the Schgr-NPF-precursor encoding transcript occurs throughout the central nervous system with relatively high transcript levels in the brain, optic lobes and suboesophageal ganglion. It was also detected at relatively high levels in the midgut, which suggests that the encoded peptide also functions in the digestive system. Moreover, Schgr-NPF-transcript levels were notably higher in starved animals than in animals fed ad libitum, while transcript levels were also shown to be regulated after the consumption of a meal. Injection of locust trNPF in adults stimulated food intake, while RNAi knockdown reduced food intake. Furthermore, injection of trNPF in adults stimulated weight increase, while RNAi knockdown reduced weight gain. This effect of trNPF on body weight gain may result from its stimulatory effect on food intake. Taken together, we provide clear evidence for an important role of trNPF in the regulation of feeding in the desert locust, S. gregaria. (C) 2012 Elsevier Ltd. All rights reserved. |
英文关键词 | Insect Hormone Regulatory peptide Food intake Weight gain |
类型 | Article |
语种 | 英语 |
国家 | Belgium |
收录类别 | SCI-E |
WOS记录号 | WOS:000313934800011 |
WOS关键词 | Y-LIKE ; MIGRATORY LOCUST ; INSULIN-LIKE ; RHODNIUS-PROLIXUS ; ANOPHELES-GAMBIAE ; ADULT DEVELOPMENT ; NERVOUS-SYSTEM ; DROSOPHILA ; PEPTIDES ; IDENTIFICATION |
WOS类目 | Biochemistry & Molecular Biology ; Entomology |
WOS研究方向 | Biochemistry & Molecular Biology ; Entomology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/177649 |
作者单位 | Katholieke Univ Leuven, Inst Zool, Dept Anim Physiol & Neurobiol, Mol Dev Physiol & Signal Transduct, B-3000 Louvain, Belgium |
推荐引用方式 GB/T 7714 | Van Wielendaele, Pieter,Dillen, Senne,Zels, Sven,et al. Regulation of feeding by Neuropeptide F in the desert locust, Schistocerca gregaria[J],2013,43(1):102-114. |
APA | Van Wielendaele, Pieter,Dillen, Senne,Zels, Sven,Badisco, Liesbeth,&Vanden Broeck, Jozef.(2013).Regulation of feeding by Neuropeptide F in the desert locust, Schistocerca gregaria.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,43(1),102-114. |
MLA | Van Wielendaele, Pieter,et al."Regulation of feeding by Neuropeptide F in the desert locust, Schistocerca gregaria".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 43.1(2013):102-114. |
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