Arid
DOI10.3109/08958378.2013.797524
Assessment of geographical variation in the respiratory toxicity of desert dust particles
Taylor, Karen1; Foster, Melanie L.1; Law, J. McHugh1; Centeno, Jose A.2; Fornero, Elisa2; Henderson, M. Stephen1; Trager, Sabrina A.1; Stockelman, Michael G.3; Dorman, David C.1
通讯作者Dorman, David C.
来源期刊INHALATION TOXICOLOGY
ISSN0895-8378
出版年2013
卷号25期号:7页码:405-416
英文摘要

The health consequences of sand particle inhalation are incompletely understood. This project evaluated the respiratory toxicity of sand particles collected at military bases near Fort Irwin USA, in Iraq (Camp Victory, Taji and Talil), and Khost Afghanistan. Our primary focus was on assessing the role of soluble metals in the respiratory toxicity of the sand particles using in vitro and in vivo methods. Replicating rat type II alveolar cell cultures (RLE-6TN) were exposed to sand extracts or vehicle control in serum-free media for <= 24 h. Cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and assessment of lactate dehydrogenase leakage. The relative in vitro cytotoxicity of the sand extracts was Taji approximate to Talil > Afghanistan > Camp Victory approximate to Fort Irwin. We also assessed extracts of Camp Victory, Afghanistan, and Taji sand for acute and delayed pulmonary toxicity in rats following intratracheal administration. Assessments included biochemical analysis of bronchoalveolar lavage fluid (BALF) and lung histopathology. The in vitro cytotoxicity assay results were partially predictive of in vivo responses. The more cytotoxic Taji sand extract induced an acute irritant response in rats following intratracheal administration. Rats given the less cytotoxic Camp Victory sand extract had minimal biochemical or cytological BALF changes whereas rats given either the Afghanistan or Taji sand extracts demonstrated BALF changes that were suggestive of mild lung inflammation. Unexpectedly, we observed similar lung pathology in all extract-exposed rats. The results of our study can be used to prioritize future particle inhalation studies or guide epidemiological study design.


英文关键词Cytotoxicity dust inhalation in vitro in vivo particle toxicity pulmonary toxicity rat
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000320860300005
WOS关键词OIL FLY-ASH ; PULMONARY HOST-DEFENSE ; NECROSIS-FACTOR-ALPHA ; COPPER SMELTER DUST ; EX-VIVO PRODUCTION ; AL ESKAN DISEASE ; MILITARY PERSONNEL ; PARTICULATE MATTER ; LUNG INJURY ; AMBIENT AIR
WOS类目Toxicology
WOS研究方向Toxicology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/177645
作者单位1.N Carolina State Univ, Coll Vet Med, Dept Mol Biomed Sci, Raleigh, NC 27606 USA;
2.Joint Base Andrews Naval Air Facil, Malcolm Grow Med Clin, Biophys Toxicol Lab, Joint Pathol Ctr,Div Biophys Toxicol, Baltimore, MD USA;
3.Naval Res Lab, Ctr Biomol Sci & Engn, Washington, DC USA
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GB/T 7714
Taylor, Karen,Foster, Melanie L.,Law, J. McHugh,et al. Assessment of geographical variation in the respiratory toxicity of desert dust particles[J],2013,25(7):405-416.
APA Taylor, Karen.,Foster, Melanie L..,Law, J. McHugh.,Centeno, Jose A..,Fornero, Elisa.,...&Dorman, David C..(2013).Assessment of geographical variation in the respiratory toxicity of desert dust particles.INHALATION TOXICOLOGY,25(7),405-416.
MLA Taylor, Karen,et al."Assessment of geographical variation in the respiratory toxicity of desert dust particles".INHALATION TOXICOLOGY 25.7(2013):405-416.
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