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Simultaneous determination of five alkaloids (morphine, codeine, noscapine, thebaine, and papaverine) of pericarpium papaveris in hot pot broth was established by ultra-performance liquid chromatography (UPLC) coupled to triple quadruple mass spectrometry (QqQ-MS). Water containing 0.1 mol/L hydrochloric acid was used as extraction solution and petroleum ether were used for fat removal. Then, the water-phase extracts were purified by an Oasis MCX cartridge. After separation by a BEH C18 (50 x 2.1 mm, 1.7 mu m) column, five alkaloids were detected by mass spectrometry in the positive electrospray ionization with multiple reaction monitoring (MRM). The linear ranges were 0.1-10.0 mu g/kg for morphine, codeine, and thebaine, as well as 0.01-1.0 mu g/kg for papaverine and noscapine, respectively. The limits of quantification (LOQs) for morphine, codeine, and thebaine were 0.1 mu g/kg and for papaverine and noscapine were 0.01 mu g/kg. Mean recoveries at three spiked concentrations for the objectives were varied from 72.1 % to 124 %. The method was employed in real samples and demonstrated its rapidness and high sensibility.