Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s00792-012-0510-8 |
Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics | |
Bhat, Archana1; Riyaz-Ul-Hassan, Syed1; Ahmad, Nasier1; Srivastava, Nidhi2; Johri, Sarojini1 | |
通讯作者 | Johri, Sarojini |
来源期刊 | EXTREMOPHILES
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ISSN | 1431-0651 |
EISSN | 1433-4909 |
出版年 | 2013 |
卷号 | 17期号:2页码:229-239 |
英文摘要 | Mining of soil sample from cold desert of Ladakh by functional metagenomics led to the isolation of cold-adapted endocellulase (CEL8M) that hydrolyses carboxymethyl cellulose (CMC). Mature CEL8M, a 347-residue polypeptide with a molecular mass of 38.9 kDa showed similarity to beta-1,3-1,4 d-glucanase from Klebsiella sp. The enzyme contains the catalytic module of glycosyl hydrolase family 8 but does not possess a carbohydrate-binding domain. 3D structural model of the enzyme built by homology modeling showed an architecture of (alpha/alpha)(6)-barrel fold. The purified enzyme was found to be active against CMC, xylan, colloidal chitosan and lichenan but not active against avicel. Glucose was not among the initial hydrolysis products, indicating an endo mode of action. CEL8M displayed maximal activity at pH 4.5 and remained significantly active (similar to 28 %) when the temperature decreased to 10 A degrees C. Cold-active endocellulase CEL8M may find applications in textile industry at low temperature which can result in energy savings. |
英文关键词 | Ladakh Metagenomic Cold-active protein pH profile Endoglucanase |
类型 | Article |
语种 | 英语 |
国家 | India |
收录类别 | SCI-E |
WOS记录号 | WOS:000315574600004 |
WOS关键词 | ENVIRONMENTAL DNA LIBRARIES ; ESCHERICHIA-COLI ; CELLULASE GENES ; PURIFICATION ; MICROORGANISMS ; BACTERIA ; CLONING ; ENDOGLUCANASES ; CONSTRUCTION ; CHITOSANASE |
WOS类目 | Biochemistry & Molecular Biology ; Microbiology |
WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/177103 |
作者单位 | 1.Indian Inst Integrat Med, Microbial Biotechnol Div, Jammu 180001, India; 2.Banasthali Univ, Dept Biosci & Biotechnol, Jaipur, Rajasthan, India |
推荐引用方式 GB/T 7714 | Bhat, Archana,Riyaz-Ul-Hassan, Syed,Ahmad, Nasier,et al. Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics[J],2013,17(2):229-239. |
APA | Bhat, Archana,Riyaz-Ul-Hassan, Syed,Ahmad, Nasier,Srivastava, Nidhi,&Johri, Sarojini.(2013).Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics.EXTREMOPHILES,17(2),229-239. |
MLA | Bhat, Archana,et al."Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics".EXTREMOPHILES 17.2(2013):229-239. |
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