Arid
DOI10.1007/s00792-012-0510-8
Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics
Bhat, Archana1; Riyaz-Ul-Hassan, Syed1; Ahmad, Nasier1; Srivastava, Nidhi2; Johri, Sarojini1
通讯作者Johri, Sarojini
来源期刊EXTREMOPHILES
ISSN1431-0651
EISSN1433-4909
出版年2013
卷号17期号:2页码:229-239
英文摘要

Mining of soil sample from cold desert of Ladakh by functional metagenomics led to the isolation of cold-adapted endocellulase (CEL8M) that hydrolyses carboxymethyl cellulose (CMC). Mature CEL8M, a 347-residue polypeptide with a molecular mass of 38.9 kDa showed similarity to beta-1,3-1,4 d-glucanase from Klebsiella sp. The enzyme contains the catalytic module of glycosyl hydrolase family 8 but does not possess a carbohydrate-binding domain. 3D structural model of the enzyme built by homology modeling showed an architecture of (alpha/alpha)(6)-barrel fold. The purified enzyme was found to be active against CMC, xylan, colloidal chitosan and lichenan but not active against avicel. Glucose was not among the initial hydrolysis products, indicating an endo mode of action. CEL8M displayed maximal activity at pH 4.5 and remained significantly active (similar to 28 %) when the temperature decreased to 10 A degrees C. Cold-active endocellulase CEL8M may find applications in textile industry at low temperature which can result in energy savings.


英文关键词Ladakh Metagenomic Cold-active protein pH profile Endoglucanase
类型Article
语种英语
国家India
收录类别SCI-E
WOS记录号WOS:000315574600004
WOS关键词ENVIRONMENTAL DNA LIBRARIES ; ESCHERICHIA-COLI ; CELLULASE GENES ; PURIFICATION ; MICROORGANISMS ; BACTERIA ; CLONING ; ENDOGLUCANASES ; CONSTRUCTION ; CHITOSANASE
WOS类目Biochemistry & Molecular Biology ; Microbiology
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/177103
作者单位1.Indian Inst Integrat Med, Microbial Biotechnol Div, Jammu 180001, India;
2.Banasthali Univ, Dept Biosci & Biotechnol, Jaipur, Rajasthan, India
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GB/T 7714
Bhat, Archana,Riyaz-Ul-Hassan, Syed,Ahmad, Nasier,et al. Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics[J],2013,17(2):229-239.
APA Bhat, Archana,Riyaz-Ul-Hassan, Syed,Ahmad, Nasier,Srivastava, Nidhi,&Johri, Sarojini.(2013).Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics.EXTREMOPHILES,17(2),229-239.
MLA Bhat, Archana,et al."Isolation of cold-active, acidic endocellulase from Ladakh soil by functional metagenomics".EXTREMOPHILES 17.2(2013):229-239.
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