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Livestock grazing is one of the main causes for the change of soil organic carbon (SOC) and total nitrogen (TN) in the arid and semi-arid parts in northern China. This paper examined the SOC, TN, and their components of the local steppe and desert steppe, considering continuous grazing and 4-year livestock exclusion, respectively. In steppe where livestock is excluded, both SOC and TN in the topsoil (0-0.20 m) are found to remain unchanged; however, significant growths are found in microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), particulate organic carbon (POC), and particulate organic nitrogen (PON). On the contrary, both POC and PON progressively decrease at the continuous grazing sites, attributed to the reduction of the mass proportions of soil particulate fraction in the top 0.10 m soil. In the desert steppe where grazing is excluded, the SOC, TN, and their components of the topsoil increase. However, at the continuous grazing sites, POC and PON in the 0.10 m topsoil are reduced, caused by the decrease of C and N content in soil particulate fraction. Besides, microbial quotients were lower in the continuous grazing sites in the two grasslands. It is also found that both MBC and POC are more sensitive to human-induced activities than SOC, and thus could serve as earlier indicators of the soil-fertility variation caused by short-period grazing management.