干旱区生态环境知识资源中心

Germination efficiency of freshly harvested Hippophae rhamnoides seeds collected from cold desert of Ladakh outside its native place under in vivo glass house condition recorded a highest of 73% germination in soilrite at 22 +/- 2 degrees C and 60-70% RH within three weeks at Guwahati, North East India. Germination of seeds was significantly enhancedto a maximum of 90% under in vitro condition in 1/4th Murashige & Skoog (MS) medium supplemented with 3% sucrose and 2% activated charcoal within two weeks. Glass house acclimatized healthy seedlings after been introduced to natural climatic conditions of Shillong and Guwahati of North East India showed inadequate survivability. Unlike storage at room temperature which is detrimental for seed viability, low temperature storage under refrigeration and natural climatic condition of Ladakh retains viability for prolonged period.