Arid
DOI10.1016/j.cryobiol.2012.11.005
A novel function - Thermal protective properties of an antifreeze protein from the summer desert beetle Microdera punctipennis
Qiu, Liming; Mao, Xinfang; Hou, Feng; Ma, Ji
通讯作者Ma, Ji
来源期刊CRYOBIOLOGY
ISSN0011-2240
出版年2013
卷号66期号:1页码:60-68
英文摘要

It is well known that antifreeze proteins play an important role in protecting poikilothermic organisms from freezing. However, the transcripts of antifreeze protein genes, Mpafps, were observed in the desert beetle Microdera punctipennis in summer. The mRNA levels of Mpafps increased significantly after heat shock at 50 degrees C, which implies that a novel function may exist in the antifreeze proteins from M. punctipennis. Southern blot analysis suggested the presence of multiple copies of the Mpafps family in the genome. Transcripts of two cDNAs encoding antifreeze proteins (Mpafps77 and Mpafps52) were isolated from beetles collected in the summer. The deduced amino acid sequences of the MpAFPs expressed in the summer are shorter by one 12-residue repeat and have significantly different C-terminal end sequences relative to the AFPs expressed in winter. Mpafps77 was constructed and expressed in Escherichia colt as a fusion protein, maltose-binding protein (MBP)-MpAFPS77. An in vitro heat protection assay was done by measuring the survival of bacteria and yeast that were exposed to 50 and 42 degrees C, respectively and showed that the fusion protein significantly increased the thermal tolerance of these cells. It also increased the thermotolerance of the lactate dehydrogenase (LDH) enzyme at 65 degrees C. These studies are the first biochemical demonstration of a thermal protective function for MpAFP and suggest some novel protective mechanisms may be present in M. punctipennis. (C) 2012 Elsevier Inc. All rights reserved.


英文关键词Insect antifreeze protein Thermal hysteresis Thermal protective properties Water conservation Microdera punctipennis
类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000315544100011
WOS关键词TENEBRIO-MOLITOR ; TERRESTRIAL ARTHROPODS ; CHORISTONEURA-FUMIFERANA ; DENDROIDES-CANADENSIS ; ICE ; WATER ; INSECTS ; LARVAE ; GLYCOPEPTIDES ; TRANSCRIPTION
WOS类目Biology ; Physiology
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Physiology
来源机构新疆大学
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/176551
作者单位Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China
推荐引用方式
GB/T 7714
Qiu, Liming,Mao, Xinfang,Hou, Feng,et al. A novel function - Thermal protective properties of an antifreeze protein from the summer desert beetle Microdera punctipennis[J]. 新疆大学,2013,66(1):60-68.
APA Qiu, Liming,Mao, Xinfang,Hou, Feng,&Ma, Ji.(2013).A novel function - Thermal protective properties of an antifreeze protein from the summer desert beetle Microdera punctipennis.CRYOBIOLOGY,66(1),60-68.
MLA Qiu, Liming,et al."A novel function - Thermal protective properties of an antifreeze protein from the summer desert beetle Microdera punctipennis".CRYOBIOLOGY 66.1(2013):60-68.
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