干旱区生态环境知识资源中心(Arid): Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing

Arid

DOI	10.1007/s12686-012-9842-z
	Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing
	Alfadala, Sanad 1; Dawson, Deborah A.1; Horsburgh, Gavin J.1; Behnke, Jerzy M.2; Bajer, Anna 3; Mohallal, Eman M. E.4; Zalat, Samy 5,6; Slate, Jon 1
通讯作者	Alfadala, Sanad
来源期刊	CONSERVATION GENETICS RESOURCES
ISSN	1877-7252
出版年	2013
卷号	5 期号:2 页码:519-524
英文摘要	We isolated and characterized Eastern spiny mouse, Acomys dimidiatus microsatellite loci. A microsatellite-enriched library was created and A. dimidiatus fragments sequenced using 454 sequencing. In total, 1,221 primer-designable microsatellite sequences were identified. We designed primer sets for 20 loci. Loci were characterized in A. dimidiatus individuals from a semi-isolated desert wadi (valley) in St. Katherine Protectorate, Egypt. After initial trails, 18 microsatellite loci were genotyped in 67 mice. The number of alleles displayed in the 18 markers ranged from three to nine (mean = 6) with mean expected and observed heterozygosities of 0.63 and 0.65, respectively. All 18 selected loci were in Hardy-Weinberg equilibrium (P > 0.01). These markers will be used to investigate the fine-scale spatial patterns of genetic diversity and divergence of A. dimidiatus populations. The isolated loci are of potential utility in other murines, including 260 threatened species.
英文关键词	Eastern spiny mouse Pyrosequencing Microsatellite markers Muridae Rodent Simple sequence repeats (SSRs)
类型	Article
语种	英语
国家	England ; Poland ; Egypt ; Saudi Arabia
收录类别	SCI-E
WOS记录号	WOS:000318293100054
WOS类目	Biodiversity Conservation ; Genetics & Heredity
WOS研究方向	Biodiversity & Conservation ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/176523
作者单位	1.Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England; 2.Univ Nottingham, Sch Biol, Nottingham NG7 2RD, England; 3.Univ Warsaw, Inst Zool, Dept Parasitol, PL-02096 Warsaw, Poland; 4.Desert Res Ctr, Cairo, Egypt; 5.Suez Canal Univ, Dept Zool, I Smailia, Egypt; 6.Taibah Univ, Fac Arts & Sci, El Ula Branch, El Ula, Saudi Arabia
推荐引用方式 GB/T 7714	Alfadala, Sanad,Dawson, Deborah A.,Horsburgh, Gavin J.,et al. Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing[J],2013,5(2):519-524.
APA	Alfadala, Sanad.,Dawson, Deborah A..,Horsburgh, Gavin J..,Behnke, Jerzy M..,Bajer, Anna.,...&Slate, Jon.(2013).Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing.CONSERVATION GENETICS RESOURCES,5(2),519-524.
MLA	Alfadala, Sanad,et al."Large-scale isolation of Eastern spiny mouse Acomys dimidiatus microsatellite loci through GS-FLX 454 titanium sequencing".CONSERVATION GENETICS RESOURCES 5.2(2013):519-524.