Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3732/apps.1300048 |
ISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING | |
Arroyo, Juan M.1; Munguia-Vega, Adrian2,3,4; Rodriguez-Estrella, Ricardo3; Bascompte, Jordi1 | |
通讯作者 | Bascompte, Jordi |
来源期刊 | APPLICATIONS IN PLANT SCIENCES
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ISSN | 2168-0450 |
出版年 | 2013 |
卷号 | 1期号:12 |
英文摘要 | Premise of the study: Microsatellite primers were developed for the parasitic mistletoe Phoradendron californicum to investigate to what extent population genetic structure depends on host tree distribution within a highly fragmented landscape. Methods and Results: Fourteen unlinked polymorphic and four monomorphic nuclear microsatellite markers were developed using a genomic shotgun pyrosequencing method. A total of 187 alleles plus four monomorphic loci alleles were found in 98 individuals sampled in three populations from the Sonoran Desert in the Baja California peninsula (Mexico). Loci averaged 13.3 alleles per locus (range 4-28), and observed and expected heterozygosities within populations varied from 0.167-0.879 and 0.364-0.932, respectively. Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and fragmentation in natural populations of this parasitic plant. Cross-species amplifications in P. juniperinum and P. diguetianum only showed four markers that could be useful in P. diguetianum. |
英文关键词 | mistletoe parasitic plant Phoradendron californicum shotgun sequencing SSR toji |
类型 | Article |
语种 | 英语 |
国家 | Spain ; USA ; Mexico |
收录类别 | SCI-E |
WOS记录号 | WOS:000342558500002 |
WOS关键词 | POPULATION-GENETICS ; SOFTWARE ; PRIMERS ; PROGRAM ; WINDOWS ; LINUX |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
来源机构 | University of Arizona |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/175747 |
作者单位 | 1.CSIC, Dept Integrat Ecol Estac Biol Donana, E-41092 Seville, Spain; 2.Univ Arizona, Sch Nat Resources & Environm, Conservat Genet Lab, Tucson, AZ 85721 USA; 3.CIBNOR, Environm Planning & Conservat Program, La Paz 23090, Baja California, Mexico; 4.Comunidad & Biodiversidad AC, Guaymas 85420, Sonora, Mexico |
推荐引用方式 GB/T 7714 | Arroyo, Juan M.,Munguia-Vega, Adrian,Rodriguez-Estrella, Ricardo,et al. ISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING[J]. University of Arizona,2013,1(12). |
APA | Arroyo, Juan M.,Munguia-Vega, Adrian,Rodriguez-Estrella, Ricardo,&Bascompte, Jordi.(2013).ISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING.APPLICATIONS IN PLANT SCIENCES,1(12). |
MLA | Arroyo, Juan M.,et al."ISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING".APPLICATIONS IN PLANT SCIENCES 1.12(2013). |
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