Arid
DOI10.1007/s10482-013-0045-8
Streptomyces fukangensis sp nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil
Zhang, Yong-Guang1; Wang, Hong-Fei1,2; Liu, Qing3; Hozzein, Wael N.4; Wadaan, Mohammed A. M.4; Cheng, Juan3; Chen, Yue-Ji1; Zhang, Yuan-Ming1; Li, Wen-Jun1,3,4
通讯作者Li, Wen-Jun
来源期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
EISSN1572-9699
出版年2013
卷号104期号:6页码:1227-1233
英文摘要

An alkaliphilic actinobacterium, designated EGI 80050(T), was isolated from a desert soil sample of Xinjiang, north-west China, and characterized by a polyphasic approach. The isolate was observed to produce purple orange-yellow aerial mycelium and dark orange-yellow substrate mycelium on yeast extract-malt extract agar medium. Whole-cell hydrolysates of strain EGI 80050(T) were found to contain ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, rhamnose and mannose as the main sugars. The major fatty acids identified were C-16:0-iso (36.8 %), C-15:0-anteiso (17.3 %), 15:0-iso (13.2 %) and 14:0-iso (10.5 %). The predominant menaquinones detected were MK-9(H-6) and MK-9(H-8), while the characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylmethylethanolamine and three unknown phospholipids. The G+C content of the genomic DNA was determined to be 67.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain EGI 80050(T) to the genus Streptomyces. Levels of 16 rRNA gene sequence similarities between strain EGI 80050(T) and Streptomyces candidus NRRL ISP-5141(T), Streptomyces cremeus NBRC 12760(T), Streptomyces spiroverticillatus NBRC 12821(T), Streptomyces violaceorectus NBRC 13102(T), Streptomyces cinereoruber subsp. cinereoruber NBRC 12756(T) were 96.7, 96.6, 96.6, 96.6 and 96.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain EGI 80050(T) is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fukangensis sp. nov. (type strain EGI 80050(T) = BCRC 16945(T) = JCM 19127(T)) is proposed.


英文关键词Alkaliphilic actinomycete Polyphasic taxonomy Streptomyces fukangensis sp nov.
类型Article
语种英语
国家Peoples R China ; Saudi Arabia
收录类别SCI-E
WOS记录号WOS:000326804000033
WOS关键词DEOXYRIBONUCLEIC-ACID ; EMENDED DESCRIPTION ; MAXIMUM-LIKELIHOOD ; GEN. NOV. ; CLASSIFICATION ; CHROMATOGRAPHY ; CHINA ; TREES
WOS类目Microbiology
WOS研究方向Microbiology
来源机构中国科学院新疆生态与地理研究所 ; King Saud University
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/175744
作者单位1.Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Yunnan, Peoples R China;
4.King Saud Univ, Coll Sci, Bioprod Res Chair, Riyadh 11451, Saudi Arabia
推荐引用方式
GB/T 7714
Zhang, Yong-Guang,Wang, Hong-Fei,Liu, Qing,et al. Streptomyces fukangensis sp nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil[J]. 中国科学院新疆生态与地理研究所, King Saud University,2013,104(6):1227-1233.
APA Zhang, Yong-Guang.,Wang, Hong-Fei.,Liu, Qing.,Hozzein, Wael N..,Wadaan, Mohammed A. M..,...&Li, Wen-Jun.(2013).Streptomyces fukangensis sp nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,104(6),1227-1233.
MLA Zhang, Yong-Guang,et al."Streptomyces fukangensis sp nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 104.6(2013):1227-1233.
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