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In this research, temporal trends of reference evapotranspiration (ET0) values were investigated in arid regions of Iran. For this purpose, meteorological observations collected from 11 high quality meteorological sites over a 41-year period (1965-2005) were used and statistically significant ET0 trends in the monthly, seasonal and annual time basis were detected using nonparametric Mann-Kendall (MK) and Spearman’s Rho (SR) tests at the 5% significant level. To eliminate the effect of serial correlation on the test results, the Trend Free Pre-Whitening (TFPW) approach was applied. The results of this study indicated that the ET0 trends for some cities were increasing (positive) however, for some sites, they showed decreasing (negative) trends. The most number of significant ET0 trends on a monthly time scale occurred at Birjand but no significant trend was observed at the Bandarabbas, Sabzevar and Semnan sites. On the annual time scale, Mashhad revealed the highest positive trend (7.5 mm per year). On the other hand, Esfahan showed the highest decreasing (negative) ET0 trend of about -6.38 mm per year. In general, the performances and abilities of the MK and SR tests were consistent at the verified significant level.