Arid
DOI10.1016/j.quascirev.2012.10.021
High-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens
Murray, Daithi C.1; Pearson, Stuart G.2; Fullagar, Richard3; Chase, Brian M.4,5; Houston, Jayne1; Atchison, Jennifer3; White, Nicole E.1; Bellgard, Matthew I.6; Clarke, Edward7; Macphail, Mike8; Gilbert, M. Thomas P.1,9; Haile, James1; Bunce, Michael1
通讯作者Bunce, Michael
来源期刊QUATERNARY SCIENCE REVIEWS
ISSN0277-3791
出版年2012
卷号58页码:135-145
英文摘要

The study of arid palaeoenvironments is often frustrated by the poor or non-existent preservation of plant and animal material, yet these environments are of considerable environmental importance. The analysis of pollen and macrofossils isolated from herbivore middens has been an invaluable source of information regarding past environments and the nature of ecological fluctuations within arid zones. The application of ancient DNA (aDNA) techniques to hot, arid zone middens remains unexplored. This paper attempts to retrieve and characterise aDNA from four Southern Hemisphere fossil middens: three located in hot, arid regions of Australia and one sample from South Africa’s Western Cape province. The middens are dated to between 30,490 (+/- 380) and 710 (+/- 70) cal yr BP. The Brockman Ridge midden in this study is potentially the oldest sample from which aDNA has been successfully extracted in Australia. The application of high-throughput sequencing approaches to profile the biotic remains preserved in midden material has not been attempted to date and this study clearly demonstrates the potential of such a methodology. In addition to the taxa previously detected via macrofossil and palynological analyses, aDNA analysis identified unreported plant and animal taxa, some of which are locally extinct or endemic. The survival and preservation of DNA in hot, arid environments is a complex and poorly understood process that is both sporadic and rare, but the survival of DNA through desiccation may be important. Herbivore middens now present an important source of material for DNA metabarcoding studies of hot, arid palaeoenvironments and can potentially be used to analyse middens in these environments throughout Australia, Africa, the Americas and the Middle East. (C) 2012 Elsevier Ltd. All rights reserved.


英文关键词Ancient DNA Herbivore midden Palaeoenvironment Arid zone Metabarcoding High throughput sequencing
类型Article
语种英语
国家Australia ; France ; Norway ; Denmark
收录类别SCI-E ; SSCI
WOS记录号WOS:000312428400011
WOS关键词EXTINCT GROUND SLOTH ; MOLECULAR ANALYSIS ; ENVIRONMENTAL DNA ; LATE PLEISTOCENE ; GENE-SEQUENCES ; CLIMATE-CHANGE ; HYRAX MIDDENS ; WESTERN CAPE ; DIET ; VEGETATION
WOS类目Geography, Physical ; Geosciences, Multidisciplinary
WOS研究方向Physical Geography ; Geology
来源机构French National Research Institute for Sustainable Development
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/174727
作者单位1.Murdoch Univ, Ancient DNA Lab, Sch Biol Sci & Biotechnol, Murdoch, WA 6150, Australia;
2.Univ New S Wales, PaleoLab, Sch Phys Environm & Math Sci, Canberra, ACT 2610, Australia;
3.Univ Wollongong, Ctr Archaeol Sci, Sch Earth & Environm Sci, Wollongong, NSW 2522, Australia;
4.Univ Montpellier 2, Inst Sci Evolut Montpellier, UMR 5554, CNRS, F-34095 Montpellier 5, France;
5.Univ Bergen, Dept Archaeol Hist Culture & Relig, N-5020 Bergen, Norway;
6.Murdoch Univ, Ctr Comparat Genom, Murdoch, WA 6150, Australia;
7.Rio Tinto, Dampier, WA, Australia;
8.Australian Natl Univ, Dept Archaeol & Nat Hist, Coll Asia & Pacific, Canberra, ACT 0200, Australia;
9.Univ Copenhagen, Ctr GeoGenet, Nat Hist Museum Denmark, DK-1350 Copenhagen, Denmark
推荐引用方式
GB/T 7714
Murray, Daithi C.,Pearson, Stuart G.,Fullagar, Richard,et al. High-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens[J]. French National Research Institute for Sustainable Development,2012,58:135-145.
APA Murray, Daithi C..,Pearson, Stuart G..,Fullagar, Richard.,Chase, Brian M..,Houston, Jayne.,...&Bunce, Michael.(2012).High-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens.QUATERNARY SCIENCE REVIEWS,58,135-145.
MLA Murray, Daithi C.,et al."High-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens".QUATERNARY SCIENCE REVIEWS 58(2012):135-145.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Murray, Daithi C.]的文章
[Pearson, Stuart G.]的文章
[Fullagar, Richard]的文章
百度学术
百度学术中相似的文章
[Murray, Daithi C.]的文章
[Pearson, Stuart G.]的文章
[Fullagar, Richard]的文章
必应学术
必应学术中相似的文章
[Murray, Daithi C.]的文章
[Pearson, Stuart G.]的文章
[Fullagar, Richard]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。