Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11104-012-1152-4 |
Bacterial community in the rhizosphere of the cactus species Mammillaria carnea during dry and rainy seasons assessed by deep sequencing | |
Torres-Cortes, G.1; Millan, V.1; Fernandez-Gonzalez, A. J.1; Aguirre-Garrido, J. F.2; Ramirez-Saad, H. C.2; Fernandez-Lopez, M.1; Toro, N.1; Martinez-Abarca, F.1 | |
通讯作者 | Martinez-Abarca, F. |
来源期刊 | PLANT AND SOIL
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ISSN | 0032-079X |
EISSN | 1573-5036 |
出版年 | 2012 |
卷号 | 357期号:1-2页码:275-288 |
英文摘要 | The Tehuacan-Cuitcatlan reserve is an area of unique plant biodiversity mostly in the form of xerophytes, with exceptionally high numbers of rare and endemic species. This endemism results partly from the characteristics of the climate of this area, with two distinct seasons: rainy and dry seasons. Although rhizosphere communities must be critical in the function of this ecosystem, understanding the structure of these communities is currently limited. This is the first molecular study of the microbial diversity present in the rhizosphere of Mamillaria carnea. Total DNA was obtained from soil and rhizosphere samples at three locations in the Tehuacan Cuicatlan Reserve, during dry and rainy seasons. Temperature gradient gel electrophoresisis (TGGE) fingerprinting, 16S rRNA gene libraries and pyrosequencing were used to investigate bacterial diversity in the rhizosphere of Mammillaria carnea and changes in the microbial community between seasons. Deep sequencing data reveal a higher level of biodiversity in the dry season. Statistical analyses based on these data indicates that the composition of the bacterial community differed between both seasons affecting to members of the phyla Acidobacteria, Cyanobacteria, Gemmatimonadetes, Plantomycetes, Actinobacteria and Firmicutes. In addition, the depth of sequencing performed (> 24,000 reads) enables detection of changes in the relative abundance of lower bacterial taxa (novel bacterial phylotypes) indicative of the increase of specific bacterial populations due to the season. This study states the basis of the bacterial diversity in the rhizosphere of cacti in semi-arid environments and it is a sequence-based demonstration of community shifts in different seasons. |
英文关键词 | Microbial community Soil bacteria 16S rRNA analysis Mammillaria carnea Tehuacan Cuicatlan reserve 454-pyrosequencing |
类型 | Article |
语种 | 英语 |
国家 | Spain ; Mexico |
收录类别 | SCI-E |
WOS记录号 | WOS:000306552700022 |
WOS关键词 | 16S RIBOSOMAL-RNA ; MICROBIAL COMMUNITY ; SOIL ; DIVERSITY ; GENE ; DNA ; CLASSIFICATION ; CYANOBACTERIA ; AMPLIFICATION ; PATTERNS |
WOS类目 | Agronomy ; Plant Sciences ; Soil Science |
WOS研究方向 | Agriculture ; Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/174436 |
作者单位 | 1.CSIC, Estn Expt Zaidin, Dept Microbiol & Sistemas Simbiot, Granada 18008, Spain; 2.Univ Autonoma Metropolitana, Dept Sistemas Biol, Mexico City 04960, DF, Mexico |
推荐引用方式 GB/T 7714 | Torres-Cortes, G.,Millan, V.,Fernandez-Gonzalez, A. J.,et al. Bacterial community in the rhizosphere of the cactus species Mammillaria carnea during dry and rainy seasons assessed by deep sequencing[J],2012,357(1-2):275-288. |
APA | Torres-Cortes, G..,Millan, V..,Fernandez-Gonzalez, A. J..,Aguirre-Garrido, J. F..,Ramirez-Saad, H. C..,...&Martinez-Abarca, F..(2012).Bacterial community in the rhizosphere of the cactus species Mammillaria carnea during dry and rainy seasons assessed by deep sequencing.PLANT AND SOIL,357(1-2),275-288. |
MLA | Torres-Cortes, G.,et al."Bacterial community in the rhizosphere of the cactus species Mammillaria carnea during dry and rainy seasons assessed by deep sequencing".PLANT AND SOIL 357.1-2(2012):275-288. |
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